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Antioxidant, DNA protective efficacy and HPLC analysis of Annona muricata (soursop) extracts

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Abstract

Annona muricata is a naturally occurring edible plant with wide array of therapeutic potentials. In India, it has a long history of traditional use in treating various ailments. The present investigation was carried out to characterize the phytochemicals present in the methanolic and aqueous leaf extracts of A. muricata, followed by validation of its radical scavenging and DNA protection activities. The extracts were also analyzed for its total phenolic contents and subjected to HPLC analysis to determine its active metabolites. The radical scavenging activities were premeditated by various complementary assays (DRSA, FRAP and HRSA). Further, its DNA protection efficacy against H2O2 induced toxicity was evaluated using pBR322 plasmid DNA. The results revealed that the extracts were highly rich in various phytochemicals including luteolin, homoorientin, tangeretin, quercetin, daidzein, epicatechin gallate, emodin and coumaric acid. Both the extracts showed significant (p < 0.05) radical scavenging activities, while methanolic extract demonstrated improved protection against H2O2-induced DNA damage when compared to aqueous extract. A strong positive correlation was observed for the estimated total phenolic contents and radical scavenging potentials of the extracts. Further HPLC analysis of the phyto-constituents of the extracts provides a sound scientific basis for compound isolation.

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Abbreviations

% DRSA:

Percentage DPPH radical scavenging activity

% HRSA:

Percentage hydroxyl scavenging activity

AAE:

Ascorbic acid equivalence

ANOVA:

Analysis of variance

BHT:

Butylated hydroxytoluene

DPPH:

2, 2-diphenyl-1-picrylhydrazyl

EDTA:

Ethylene diamine tetraacetic acid

FRAP:

Ferric reducing antioxidant property

GAE:

Gallic acid equivalence

HPLC:

High performance liquid chromatography

ROS:

Reactive oxygen species

SD:

Standard deviation

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

TPTZ:

2, 4, 6-tripyridyl-s-triazin

TPC:

Total phenolic content

References

  • Adewole SO, Ojewole JA (2009) Protective effects of Annona muricata Linn. (Annonaceae) leaf aqueous extract on serum lipid profiles and oxidative stress in hepatocytes of streptozotocin-treated diabetic rats. Afr J Tradit Complement 6:30–41

    Google Scholar 

  • Alassane W, Yanjun Z, Christelle C, Jean-Paul B, Jean-Louis P, Bernard B (2004) Annomuricatin C, a novel cyclohexapeptide from the seeds of Annona muricata. C R Chim 7:981–988

    Article  Google Scholar 

  • Alothman M, Bhat R, Karim AA (2009) Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chem 115:785–788

    Article  CAS  Google Scholar 

  • Attaguile G, Russo A, Campisi A, Savoca F, Acquaviva R, Ragusa N, Vanella A (2000) Antioxidant activity and protective effect on DNA cleavage of extracts from Cistus incanus L. and Cistus monspeliensis L. Cell Biol Toxicol 16:83–90

    Article  CAS  Google Scholar 

  • Baskar R, Rajeswari V, Kumar TS (2007) In vitro antioxidant studies in leaves of Annona species. Indian J Exp Biol 45:480–485

    CAS  Google Scholar 

  • Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  Google Scholar 

  • Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200

    Article  CAS  Google Scholar 

  • Borris RP (1996) Natural product research. Perspective from a major Pharmaceutical company. J Ethnopharmacol 51:29–38

    Article  CAS  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lwt Food Sci Technol 28:25–30

    Article  CAS  Google Scholar 

  • Cijo GV, Naveen KDR, Rajkumar V, Suresh PK, Ashok KR (2012) Quantitative assessment of the relative antineoplastic potential of the n-butanolic leaf extract of Annona Muricata Linn. in normal and immortalized human cell lines. Asian Pac J Can Prev 13:699–705

    Article  Google Scholar 

  • Craig WJ (1999) Health-promoting properties of common herbs. Am J Clin Nutr 70:491S–499S

    CAS  Google Scholar 

  • Decker EA (1997) Phenolics: prooxidants or antioxidants? Nutr Rev 55:396–407

    Article  CAS  Google Scholar 

  • Franklin V, Yrene D, Karla C (2004) Antioxidant and scavenging activity of emodin, aloe-emodin, and rhein on free-radical and reactive oxygen species. Pharm Biol 42:342–348

    Article  Google Scholar 

  • Fridovich I (1999) Fundamental aspects of reactive oxygen species, or what’s the matter with oxygen? Ann N Y Acad Sci 893:13–18

    Article  CAS  Google Scholar 

  • Gordon MH (1990) The mechanism of antioxidant action in vitro. In: Hudson BJF (ed) Food antioxidants. App Sci, New York, pp l–18

    Google Scholar 

  • Gulcin I, Elias R, Gepdiremen A, Taoubi K, Koksal K (2009) Antioxidant secoiridoids from fringe tree (Chionanthus virginicus L.). Wood Sci Technol 43:195–212

    Article  CAS  Google Scholar 

  • Gunjan G, Rajkumar V, Ashok KR, Lazar M (2009) Therapeutic potential of polar and non-polar extracts of cyanthillium cinereum in vitro. Evid Based Compl Alt Med. doi:10.1093/ecam/nep155

    Google Scholar 

  • Gunjan G, Rajkumar V, Ashok KR, Lazar M (2010) Aqueous extract of Phyllanthus amarus inhibits chromium(VI)-induced toxicity in MDA-MB-435S cells. Food Chem Toxicol 48:396–401

    Article  Google Scholar 

  • Halliwell B, Aeschbach R, Loliger J, Aruoma OI (1995) The characterization of antioxidants. Food Chem Toxicol 33:601–617

    Article  CAS  Google Scholar 

  • Henning SM, Niu Y, Liu Y et al (2005) Bioavailability and antioxidant effect of epigallocatechin gallate administered in purified form versus as green tea extract in healthy individuals. J Nutr Biochem 16:610–616

    Article  CAS  Google Scholar 

  • Hochestein P, Atallah AS (1988) The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer. Mutat Res 202:363–375

    Article  Google Scholar 

  • Hopp DC, Alali FQ, Gu ZM, McLaughlin JL (1998) Mono-THF ring annonaceous acetogenins from Annona squamosa. Phytochemistry 47:803–809

    Article  CAS  Google Scholar 

  • Javanmardi J, Stushnoff C, Locke E, Vivanco JM (2003) Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem 83:547–550

    Article  CAS  Google Scholar 

  • Kappus H (1991) Lipid peroxidation – mechanism and biological relevance. In: Aruoma OI, Halliwell B (eds) Free radicals and food additives. Taylor and Francis, London, pp 59–75

    Google Scholar 

  • Klein SM, Cohen G, Cederbaum AI (1981) Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry 20:6006–6012

    Article  CAS  Google Scholar 

  • Molina MF, Sanchez-Reus I, Iglesias I, Benedi J (2003) Quercetin, a flavonoid antioxidant, prevents and protects against ethanol-induced oxidative stress in mouse liver. Biol Pharm Bull 26:1398–1402

    Article  CAS  Google Scholar 

  • Nilima S, Rajurkar HSM (2011) Estimation of Phytochemical content and antioxidant activity of some selected traditional indian medicinal plants. Indian J Pharm Sci 73:146–151

    Article  Google Scholar 

  • Othman A, Ismail A, Abdul NG, Adenan I (2007) Antioxidant capacity and phenolic content of cocoa beans. Food Chem 100:1523–1530

    Article  CAS  Google Scholar 

  • Pin-Der-Duh X (1998) Antioxidant activity of burdock (Arctium lappa Linne): its scavenging effect on free-radical active oxygen. J Am Oil Chem Soc 75:455–461

    Article  Google Scholar 

  • Rajdeep C, Abhishek D, Susri R, Nilendu S, Ashok K, Giri KC (2007) In vitro and in vivo reduction of sodium arsenite induced toxicity by aqueous garlic extract. Food Chem Toxicol 46:740–751

    Google Scholar 

  • Rajkumar V, Guha G, Kumar RA (2011) Antioxidant and anti-cancer potentials of Rheum emodi rhizome extracts. Evid Based Complement Alternat Med. doi:10.1093/ecam/neq048

    Google Scholar 

  • Rajkumar V, Gunjan G, Ashok KR (2012) Isolation and bioactivity evaluation of two metabolites from the methanolic extract of oroxylum indicum stem bark. Asian Pac J Trop Biomed 2:S7–S11

    Article  Google Scholar 

  • Russo A, Izzo AA, Cardile V (2001) An Indian medicinal plants as antiradicals and DNA cleavage protectors. Phytomedicine 8:125–132

    Article  CAS  Google Scholar 

  • Sakakibara H, Honda Y, Nakagawa S, Ashida H, Kanazawa K (2003) Simultaneous determination of all polyphenols in vegetables, fruits, and teas. J Agric Food Chem 51:571–581

    Article  CAS  Google Scholar 

  • Sindhu M, Emilia AT (2005) In vitro antioxidant activity and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Food Chem Toxicol 44:198–206

    Google Scholar 

  • Singh RP, Murthy KNC, Jayaprakash GK (2002) Studies on antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vivo models. J Agric Food Chem 50:81–86

    Article  CAS  Google Scholar 

  • Treas GE, Evans WC (1989) Pharmacognosy, 13th edn. Bailliere Tindall, London, pp 176–180

    Google Scholar 

  • Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117

    Article  CAS  Google Scholar 

  • Watt JM, Breyer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern and Eastern Africa, 2nd edn. Churchill Livingstone, London, UK, pp 58–59

    Google Scholar 

  • Wong C, Li H, Cheng KW, Chen F (2006) A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay. Food Chem 97:705–711

    Article  CAS  Google Scholar 

  • Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M (2002) Free radical scavenging properties of wheat extracts. J Agric Food Chem 50:1619–1624

    Article  CAS  Google Scholar 

  • Yuan SS, Chang HL, Chen HW et al (2003) Annonacin, a mono-tetrahydrofuran acetogenin, arrests cancer cells at the G1 phase and causes cytotoxicity in a Baxand caspase-3-related pathway. Life Sci 72:2853–2861

    Article  CAS  Google Scholar 

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Acknowledgment

We are thankful to the management of VIT University, Vellore, Tamil Nadu, India, for providing the necessary infrastructure for successful accomplishment of this research work. The authors also thank Mr. Biju, Teeose nursery, Mannuthy, Thrissur, Kerala, for providing the sample (leaves of Annona muricata) which is used in this study.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. School of Bio Sciences and Technology, VIT University, Vellore, India

    V. Cijo George, D. R. Naveen Kumar & P. K. Suresh

  2. Department of Zoology, Government Arts College, Dharmapuri, 636705, Tamil Nadu, India

    R. Ashok Kumar

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  1. V. Cijo George
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  2. D. R. Naveen Kumar
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  3. P. K. Suresh
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  4. R. Ashok Kumar
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Correspondence to R. Ashok Kumar.

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George, V.C., Kumar, D.R.N., Suresh, P.K. et al. Antioxidant, DNA protective efficacy and HPLC analysis of Annona muricata (soursop) extracts. J Food Sci Technol 52, 2328–2335 (2015). https://doi.org/10.1007/s13197-014-1289-7

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  • DOI: https://doi.org/10.1007/s13197-014-1289-7

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