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Isolation and extraction of antimicrobial substances against oral bacteria from lemon peel

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Abstract

We have isolated 4 antibacterial substances that were active against the oral bacteria that cause dental caries and periodontitis, such as Streptococcus mutans, Prevotella intermedia, and Porphyromonas gingivalis, from lemon peel, a waste product in the citrus industry. The isolated substances were identified as 8-geranyloxypsolaren, 5-geranyloxypsolaren, 5-geranyloxy-7-methoxycoumarin, and phloroglucinol 1-β-D-glucopyranoside (phlorin) upon structural analyses. Among these, 8-Geranyloxypsolaren, 5-geranyloxypsolaren, and 5-geranyloxy-7-methoxycoumarin exhibited high antibacterial activity. These 3 compounds were effectively extracted using ethanol and n-hexane, whereas phlorin was extracted with water. Further, the above 3 compounds were present in lemon essential oil and abundantly present in the residue produced upon the cooling treatment of concentrated lemon essential oil.

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Acknowledgment

We are grateful to Dr. Y. Sugiyama and Dr. M. Isobe of Bioagricultural Sciences, Graduate School of Nagoya University, for the FAB-MS and NMR measurements and to T. Kato and Y. Tamaki of the Pokka Corporation Ltd. for their advice on this experiment.

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Authors and Affiliations

  1. Faculty of Human Wellness, Tokaigakuen University, Miyoshi city, Aichi, 470–0207, Japan

    Yoshiaki Miyake

  2. Pokka Corporation Ltd, Kitanagoya, Aichi, 481–8515, Japan

    Masanori Hiramitsu

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  1. Yoshiaki Miyake
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  2. Masanori Hiramitsu
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Correspondence to Yoshiaki Miyake.

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Miyake, Y., Hiramitsu, M. Isolation and extraction of antimicrobial substances against oral bacteria from lemon peel. J Food Sci Technol 48, 635–639 (2011). https://doi.org/10.1007/s13197-011-0330-3

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  • DOI: https://doi.org/10.1007/s13197-011-0330-3

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