Abstract
In order to provide pertinent evidence of ion channel with immune response in the fish, whole cell patch-clamp technique was employed for potassium ion channel study in turbot (Scophthalmus maximus). Lymphocytes were isolated by Percoll density gradient centrifugation from peripheral blood samples, and electrophysiological characters of the channel were analyzed. In the recorded cells, activated voltage of the channels was −42.5±3.7 mV and the average peak current was 313.12±28.2 pA. The channel was identified as voltage dependent, the current was outward and it could be inhibited by 10 mmol/dm3 TEA or 5 mmol/dm3 4-AP, a specific potassium channel inhibitor, identifying the existence of potassium channel in peripheral lymphocytes of the turbot.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
DeCoursey T E, Chandy K G, Gupta S, et al. 1984. Voltage-gated K+ channels in human T lymphocytes: a role in mitogenesis? Nature, 307(5950): 465–468
Lunden T, Bylund G. 2000. The influence of in vitro and in vivo exposure to antibiotics on mitogeninduced proliferation of lymphoid cells in rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunol, 10(5): 395–404
Matteson D R, Deutsch C. 1984. K channels in T lymphocytes: a patch clamp study using monoclonal antibody adhesion. Nature, 307(5950): 468–471
Rader R K, Kahn L E, Anderson G D, et al. 1996. T cell activation is regulated by voltage-dependent and calcium-activated potassium channels. J Immunol, 156(4): 1425–1430
Richard N, Morrison A, Lyons B, et al. 2004. Snapper (Pagrus auratus) leucocyte proliferation is synergistically enhanced by simultaneous stimulation with LPS and PHA. Fish & Shellfish Immunology, 16(3):307–319
Stafford J L, Galvez F, Goss G, et al. 2002. Induction of nitric oxide and respiratory burst response in activated goldfish macrophages requires potassium channel activity. Dev Comp Immunol, 26(5): 445–459
Sun Xiaoxia, Song Hongchen, Zhou Qingwei, et al. 2001. Property analysis of voltage-dependent potassium channel in human peripheral blood lymphocytes and its subset. J N Bethune Univ Med Sci (in Chinese), 27(1): 22–24
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation item: The National Natural Science Foundation of China under contract Nos 40476060 and 40876089; the State Key Basic Research Program under contract No. 2006CB101805.
Rights and permissions
About this article
Cite this article
Chai, Y., Huang, X., Cong, B. et al. Potassium channel in peripheral blood lymphocytes of turbot (Scophthalmus maximus). Acta Oceanol. Sin. 29, 116–119 (2010). https://doi.org/10.1007/s13131-010-0014-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13131-010-0014-2