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Characterization of 29 highly polymorphic microsatellite markers for golden snub-nosed monkey (Rhinopithecus roxellana)

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Abstract

We isolated 29 polymorphic genomic microsatellite in golden snub-nosed monkey (Rhinopithecus roxellana) which is endemic to China. The number of alleles per locus is 5.034 ± 1.349 (mean ± SD), the observed and expected heterozygosity is 0.624 ± 0.116 and 0.614 ± 0.098, respectively. Seventeen loci were highly polymorphic (PIC > 0.5), and polymorphic information content is 0.549 ± 0.105. Suggesting a high level of genetic diversity of R. roxellana in the four locations. Four loci deviated from Hardy–Weinberg equilibrium. These markers will provide useful tools for population genetic studies of R. roxellana.

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References

  • Hao YL, Liu ZJ, Wu H, Ren BP, Wei FW, Li M (2006) Isolation and characterization of 11 microsatellite loci for the sichuan snub-nosed monkey, Rhinopithecus roxellana. Conserv Genet 8:1021–1024

    Article  Google Scholar 

  • Heather A, McCafferty DJ, Saccheri I, Alan M (2006) Non-invasive genetic sampling of the eurasian otter (Lutra luyra) using hairs. Hystrix Ital J Mammal 17:65–77

    Google Scholar 

  • Huang K, Guo ST, Cushman SA, Dunn DW, Qi XG, Hou R, Zhang J, Li Q, Zhang Q, Shi Z, Zhang K, Li BG (2016) Population structure of the golden snub-nosed monkey Rhinopithecus roxellana in the Qinling Mountains, central China. Integr Zool 11:350–360

    Article  PubMed  Google Scholar 

  • IUCN (2013) The IUCN red list of threatened species.

  • Kalinowsk ST, Taper ML, Marshall TC (2007) Revising how the computer program Cervus accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106

    Article  Google Scholar 

  • Li BG, Jia ZY, Pan RL, Ren BP (2003) Changes in distribution of the snub-nosed monkey in China. In:Marsh LK (ed) Primates in fragments: ecology and conservation. Springer, Boston. pp. 29–51.

    Chapter  Google Scholar 

  • Pan D, Li Y, Hu HX, Meng SJ, Men ZM, Fu YX, Zhang YP (2005) Microsatellite polymorphisms of sichuan golden monkeys. Chin Sci Bull 50:2850–2855

    Article  Google Scholar 

  • Qi XG, Li BG, Garber PA, Ji WH, Watanabe K (2009) Social dynamics of the golden snub-nosed monkey (Rhinopithecus roxellana): female transfer and one-male unit succession. Am J Primatol 71:670–679

    Article  PubMed  Google Scholar 

  • Qi XG, Garber PA, Ji WH, Huang ZP, Huang K, Zhang P, Guo ST, Wang XW, He G, Zhang P, Li BG (2014) Satellite telemetry and social modeling offer new insights into the origin of primate multilevel societies. Nat commun 5:5296–5306

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer 3 on the www for general users and for biologist programmers. Methods Mol Biol 132:365–386

    CAS  PubMed  Google Scholar 

  • Wang CL, Wang XW, Qi XG, Guo S, Zhao HT, Wei W, Li BG (2014) Influence of human activities on the historical and current distribution of sichuan snub-nosed monkeys in the Qinling Mountains, China. Folia Primatol 85:343–357

    Article  PubMed  Google Scholar 

  • Zhou XM, Wang BS, Pan Q, Zhang JB, Kumar S, Sun XQ, Liu ZJ, Pan HJ, Lin Y, Liu GG, Zhan W, Li MZ, Ren BP, Ma XY, Ruan H, Cheng C, Wang DW, Shi FL, Hui YY, Tao YJ, Zhang CL, Zhu PF, Xiang ZF, Jiang WK, Chang J, Wang HL, Cao ZS, Jiang Z, Li BG, Yang G, Roos C, Garber PA, Bruford MW, Li RQ, Li M (2014) Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history. Nat Genet 46:1303–1310

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This study was funded by the National Science Foundation of China (31130061, 31270438, 31470455 and 31501872), Outstanding Youth Program of National Natural Science Foundation of China (31622053), National Key Programme of Research and Development, Ministry of Science and Technology of China (2016YFC0503200). We thank Northwest University students WJ Guo, JC Sun and SY Zhang for their field work assistance and laboratory work assistance.

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Authors and Affiliations

  1. College of Life Sciences, Northwest University, Xi’an, 710069, China

    Yan-Mei Duan, Kang Huang, Xiao-Guang QI & Bao-Guo LI

  2. Shaanxi Key Laboratory for Animal Conservation, Northwest University, Xi’an, 710069, China

    Yan-Mei Duan, Kang Huang, Xiao-Guang QI & Bao-Guo LI

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  1. Yan-Mei Duan
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  2. Kang Huang
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  3. Xiao-Guang QI
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  4. Bao-Guo LI
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Corresponding authors

Correspondence to Xiao-Guang QI or Bao-Guo LI.

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Yan-Mei Duan and Kang Huang have contributed equally to this work.

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Duan, YM., Huang, K., QI, XG. et al. Characterization of 29 highly polymorphic microsatellite markers for golden snub-nosed monkey (Rhinopithecus roxellana). Conservation Genet Resour 9, 229–232 (2017). https://doi.org/10.1007/s12686-016-0658-0

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  • DOI: https://doi.org/10.1007/s12686-016-0658-0

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