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Isolation and characterization of 20 microsatellite markers for the crab-eating frog Fejervarya cancrivora

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Abstract

We isolated 20 polymorphic microsatellite markers in the crab-eating frog Fejervarya cancrivora which has experienced drastic population declines in China. The number of alleles per locus ranged from 3 to 17, and the observed heterozygosity ranged from 0.181 to 0.939. Fourteen loci were highly polymorphic (PIC > 0.5), four are moderately polymorphic (0.2 < PIC < 0.5), and two loci showed low polymorphism (PIC < 0.2). The combined probability of identity for the 20 loci was 6.2 × 10−19. Two loci deviated from Hardy–Weinberg equilibrium. These markers will provide useful tools for population genetic studies of F. cancrivora.

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Acknowledgments

This work was supported by the 985 research fund of Peking University to M. Y. We thank Y. Qiao, C. Li, F. Luo and K. Zhang for their assistance with sample collection.

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Authors and Affiliations

  1. School of Life Sciences, Peking University, Beijing, 100871, China

    Yitao Zheng & Meng Yao

  2. College of Life Sciences, Hainan Normal University, Haikou, 571158, Hainan, China

    Lijun Wang & Meiling Hong

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  1. Yitao Zheng
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  2. Lijun Wang
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  3. Meiling Hong
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  4. Meng Yao
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Correspondence to Meng Yao.

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Zheng, Y., Wang, L., Hong, M. et al. Isolation and characterization of 20 microsatellite markers for the crab-eating frog Fejervarya cancrivora . Conservation Genet Resour 8, 251–253 (2016). https://doi.org/10.1007/s12686-016-0546-7

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  • DOI: https://doi.org/10.1007/s12686-016-0546-7

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