Abstract
Using next generation sequencing and high resolution melt analyses, we identified and typed 53 SNPs in Nazca boobies (Sula granti) from the Galápagos to build a pedigree.
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References
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Acknowledgments
K. Hils, T. Stooß, C. Quinonez, and T. Schneider (University of Tübingen) are acknowledged for carrying out HRM work, and M. Lindqvuist (CEA Turku) for assistance in sequencing. We thank the Galápagos National Park Service for permission to work in the Park; the Charles Darwin Research Station, and TAME Airline. This material is based upon work supported primarily by the National Science Foundation under Grant Nos. DEB 93045679, DEB 9629539, DEB 98-06606, DEB 0235818, DEB 0842199, and DEB 1354473 to DJA.
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Thomassen, H.A., Tompkins, E.M., Thalmann, O. et al. Development and characterization of SNP markers in Nazca boobies using Ion Torrent™ sequencing and high resolution melt analysis. Conservation Genet Resour 7, 609–614 (2015). https://doi.org/10.1007/s12686-015-0458-y
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DOI: https://doi.org/10.1007/s12686-015-0458-y