Skip to main content

Advertisement

SpringerLink
Log in

Isolation and characterisation of microsatellite markers for Centrolobium tomentosum (Fabaceae), a neotropical tree species widely used for Atlantic Rainforest restoration

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

We isolated and characterised eight pairs of primers to amplify microsatellite regions for Centrolobium tomentosum, a neotropical tree species widely used for forest restoration, with important pharmacological potential. For the primer characterisation, we genotyped 48 individuals from two populations of C. tomentosum from natural remnants of Atlantic Rainforests. We detected 2–9 alleles per locus, observed and expected heterozygosities ranged from 0.08 to 0.72, and 0.08 to 0.83, respectively and we observed private alleles in six of the loci. No linkage disequilibrium was observed and all loci are in Hardy–Weinberg Equilibrium in at least one of the populations. This study presents a powerful tool for population genetic studies of this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Billotte N, Lagoda PJL, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54(4):277–288

    CAS  Google Scholar 

  • Carvalho PER (2005) Araruva. Embrapa, Colombo, p 103

    Google Scholar 

  • Goudet J (2005) Hierfstat, a package for R to compute and test hierarchical F-statistics. Mol Ecol Notes 5:184–186

    Article  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Thomas E, Jalonen R, Loo J, Boshier D, Gallo L, Cavers S, Bordács S, Smith P, Bozzano M (2014) Genetic considerations in ecosystem restoration using native tree species. Forest Ecol Manag In press

Download references

Acknowledgments

The authors would like to thank Fundação José Pedro de Oliveira, managing Mata Santa Genebra, and Instituto Florestal do Estado de São Paulo, managing Estação Ecológica de Caetetus, for the permission to collect C. tomentosum in both reservations. The work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (Biota/FAPESP-2011/50296-8; 2012/03246-8).

Author information

Authors and Affiliations

  1. Departamento de Genética, Evolução e Bioagentes, Universidade Estadual de Campinas, Campinas, Brazil

    Patricia Sanae Sujii, Kaiser Dias Schwarcz, Carolina Grando & Jaqueline Bueno de Campos

  2. Centro de Fitossanidade, Entomologia, Instituto Agronomico de Campinas, Campinas, SP, Brazil

    Giuliana Etore do Valle

  3. Departamento de Genética, ESALQ/USP, Piracicaba, Brazil

    Jose Baldin Pinheiro

  4. Agência Paulista de Tecnologia dos Agronegócios, Pólo Centro-Sul, Piracicaba, SP, Brazil

    Maria Imaculada Zucchi

Authors
  1. Patricia Sanae Sujii
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kaiser Dias Schwarcz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carolina Grando
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Giuliana Etore do Valle
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jaqueline Bueno de Campos
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jose Baldin Pinheiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Maria Imaculada Zucchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Patricia Sanae Sujii.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sujii, P.S., Schwarcz, K.D., Grando, C. et al. Isolation and characterisation of microsatellite markers for Centrolobium tomentosum (Fabaceae), a neotropical tree species widely used for Atlantic Rainforest restoration. Conservation Genet Resour 7, 733–734 (2015). https://doi.org/10.1007/s12686-015-0448-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-015-0448-0

Keywords

Search

Navigation