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Development of microsatellite markers in Moldenhawera blanchetiana and their transferability to M. luschnathiana (Fabaceae)

  • Microsatellite Letters
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Abstract

The first microsatellite (SSR) markers were developed for Moldenhawera blanchetiana and transferred to M. luschnathiana, two species endemic to the Atlantic Forest, Brazil. Sixteen loci for M. blanchetiana were characterized, 15 of which showed high levels of heterozygosity (H E  = 0.645 and H O  = 0.559). The fifteen loci were transferred to M. luschnathiana where 14 were polymorphic (H E  = 0.567 and H O  = 0.550). The average numbers of alleles per locus was 6.6 for M. blanchetiana and 4.5 for M. luschnathiana. High values of the combined exclusion probability (Q > 0.999, for both species) demonstrated the potential of these loci for paternity testing. These microsatellite markers are useful tools for performing genetic and evolutionary studies as well as for the management and conservation of populations of these two species.

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Acknowledgments

We thank José Lima da Paixão, Alesandro Souza, and Rodrigo Bezerra for support in the field, Instituto Chico Mendes de Biodiversidade (ICMBio) for providing sampling authorizations, Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for research funding (SISBIOTA Grant# PES0051/2011), and FAPESB and CNPq for scholarships to CVV, FAG, CB, and LPQ.

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Authors and Affiliations

  1. Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna Km 16, Salobrinho, Ilhéus, BA, 45662-900, Brazil

    Caio Vinicius Vivas & Fernanda Amato Gaiotto

  2. Departamento de Ciências Biológicas, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil

    Cássio van den Berg & Luciano Paganucci de Queiroz

Authors
  1. Caio Vinicius Vivas
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  2. Cássio van den Berg
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  3. Luciano Paganucci de Queiroz
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  4. Fernanda Amato Gaiotto
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Corresponding author

Correspondence to Fernanda Amato Gaiotto.

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Vivas, C.V., van den Berg, C., de Queiroz, L.P. et al. Development of microsatellite markers in Moldenhawera blanchetiana and their transferability to M. luschnathiana (Fabaceae). Conservation Genet Resour 7, 255–257 (2015). https://doi.org/10.1007/s12686-014-0351-0

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  • DOI: https://doi.org/10.1007/s12686-014-0351-0

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