Skip to main content

Advertisement

SpringerLink
Log in

Novel polymorphic EST-derived microsatellite markers for the red-listed five needle pine, Pinus dabeshanensis

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Pinus dabeshanensis is an endangered five needle pine endemic to mid-South of China. This pine finds its very limited natural occurrence in the Dabie mountains area with altitudes ranging 900–1400 m. We identified 3,516 SSR loci from the transcriptome assembly of the congeneric species P. lambertiana, and evaluated 671 loci we found. Finally, 28 novel polymorphic microsatellite markers were developed for P. dabeshanensis. Markers were characterized using 36 individuals, representing all existing populations from across the species geographic range. Species level analysis of these 28 polymorphic markers revealed the mean number per locus was 2.786 (range 2–6) and mean observed and expected heterozygosities were 0.332 (range 0.000–1.000) and 0.316 (range 0.033–0.691), respectively. This study provides the first set of microsatellite markers for the conservation genetics which in turn will facilitate conservation management for this pine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Amos W, Hoffman J, Frodsham A, Zhang L, Best S, Hill A (2007) Automated binning of microsatellite alleles: problems and solutions. Mol Ecol Notes 7(1):10–14

    Article  CAS  Google Scholar 

  • Conifer Specialist Group (1998) Pinus dabeshanensis. IUCN Red List of Threatened Species. Version 2012.1 International Union for Conservation of Nature

  • Liu L, Hao ZZ, Liu YY, Wei XX, Cun YZ, Wang XQ (2014) Phylogeography of Pinus armandii and Its Relatives: heterogeneous contributions of geography and climate changes to the genetic differentiation and diversification of Chinese white pines. PLoS One 9(1):e85920

    Article  PubMed Central  PubMed  Google Scholar 

  • Lorenz WW, Ayyampalayam S, Bordeaux JM, Howe GT, Jermstad KD, Neale DB, Rogers DL, Dean JF (2012) Conifer DBMagic: a database housing multiple de novo transcriptome assemblies for 12 diverse conifer species. Tree Genet Genomes 8(6):1477–1485

    Article  Google Scholar 

  • Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295

    Article  Google Scholar 

Download references

Acknowledgments

This research was financially supported by Grants from the “Beijing Higher Education Young Elite Teacher Project (No. YETP0756)”, the Project of the Field Ambulance and Breeding of Rare and Endan-gered Species (2012-LYSJWT-12) of the State Forestry Administration, and the Fundamental Research Funds for the Central Universities (No. YX2013-41).

Author information

Authors and Affiliations

  1. College of Landscape Architecture, National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing, 100083, China

    Zhi-Yong Zhang

  2. Department of Biology and Chemistry, Baoding University, Baoding, 071000, Hebei, China

    Bin-Bin Cui

  3. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Qinghua East Road No. 35, Beijing, 100083, People’s Republic of China

    Jian-Feng Mao, Xiao-Ming Pang, Hui Liu & Ying-Yue Li

Authors
  1. Zhi-Yong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bin-Bin Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jian-Feng Mao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiao-Ming Pang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ying-Yue Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ying-Yue Li.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 226 kb)

Supplementary material 2 (PDF 322 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, ZY., Cui, BB., Mao, JF. et al. Novel polymorphic EST-derived microsatellite markers for the red-listed five needle pine, Pinus dabeshanensis . Conservation Genet Resour 7, 191–192 (2015). https://doi.org/10.1007/s12686-014-0329-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0329-y

Keywords

Search

Navigation