Abstract
The world’s smallest penguin, Eudyptula minor, exhibits substantial mtDNA differentiation among populations, suggesting the possibility of multiple taxa. We developed 20 new microsatellite loci that potentially provide a powerful system for assessing nuclear genetic differentiation and taxonomic status of Eudyptula populations. Alleles per locus ranged from 2 to 26 and expected heterozygosity ranged from 0.317 to 0.939.
References
Banks JC, Mitchell AD, Waas JR, Paterson AM (2002) An unexpected pattern of molecular divergence within the blue penguin (Eudyptula minor) complex. Notornis 49:29–37
Benjamini Y, Hochberg Y (1995) Controlling the false discovery rate - a practical and powerful approach to multiple testing. J R Stat Soc Ser B Stat Methodol 57:289–300
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567. doi:10.1111/J.1755-0998.2010.02847.X
Faircloth BC (2008) msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94. doi:10.1111/j.1471-8286.2007.01884.x
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotech 18:233–234. doi:10.1038/72708
Acknowledgments
This research was funded by the Allan Wilson Centre, Marsden Contract UoO1112, and by a University of Otago Doctoral Scholarship.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Grosser, S., Waters, J.M. Development and characterisation of 20 novel microsatellite markers for the little blue penguin (Eudyptula minor) using next-generation sequencing. Conservation Genet Resour 7, 143–145 (2015). https://doi.org/10.1007/s12686-014-0313-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0313-6