Abstract
Hemibarbus labeo is a valuable freshwater fish species distributed in the Heilongjiang River basin in China. Wild resources have declined sharply in recent years because of overfishing and pollution. Herein, twenty polymorphic microsatellites were isolated from an enriched genomic library in H. labeo for genetic conservation. These markers were characterized in 27 wild and 48 cultured individuals. The number of alleles per locus ranged from two to ten, with an average of 5.200. The observed heterozygosity and the expected heterozygosity varied from 0.086 to 0.870 (mean = 0.538) and from 0.116 to 0.861 (mean = 0.5441), respectively. Three and two loci significantly deviated from Hard–Weinberg equilibrium in wild and cultured individuals, respectively. Three pairwise comparisons of six loci showed significant genotypic linkage disequilibrium in cultured individuals. These polymorphic markers represent a powerful tool to study population genetic diversity, genetic conservation and breeding systems in H. labeo.
References
Geng LW, Lu CY, Tong GX, Li C, Xu W (2012) Development and characterization of twenty microsatellite markers for the endangered fish Luciobarbus capito. Conserv Genet Resour 4:865–867
Lin CJ, Wang JP, Lin HD, Chiang TY (2007) Isolation and characterization of polymorphic microsatellite loci in Hemibarbus labeo (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA). Mol Ecol Notes 7:788–790
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Heredity 86:248–249
Xu W, Li CT, Geng LW, Sun HW, Liu XY (2009) Growth and reproduction of reared Hemibarbus labeo in the Wusuli River. J Fish Sci China 16:550–556
Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE 32, Microsoft windows-based freeware for population genetic analysis, version 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Alberta, Canada
Acknowledgments
This study was supported by the National Infrastructure of Fishery Germplasm Resources of China.
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Lu, C., Gu, Y., Geng, L. et al. Isolation and characterization of twenty polymorphic microsatellites for Hemibarbus labeo (Cyprinidae). Conservation Genet Resour 7, 89–92 (2015). https://doi.org/10.1007/s12686-014-0299-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-014-0299-0