Skip to main content
SpringerLink
Log in

Characterization and cross-species amplification of 19 polymorphic microsatellite loci of Hemibagrus macropterus (Bleeker) in the Yangtze River

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Hemibagrus macropterus (Bleeker) is endemic to the upper Yangtze River basin and the Zhujiang River basin in China. By using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol, we identified nineteen microsatellite loci in a wild population. The number of alleles per locus ranged from 5 to 17. The observed and expected heterozygosity ranged from 0.5132 to 1.000 and from 0.4903 to 0.9008, respectively. Cross-amplification was tested in related species Leiocassis tenuifurcatus and Pelteobagrus fulvidraco.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Ding RH (1994) The fishes of Sichuan province. Sichuan Science & Technology Press, Chengdu

    Google Scholar 

  • Li Q, Wan J (2005) SSRHunter: development of a local searching software for SSR sites. Hereditas 5:808–810 (in Chinese with English abstract)

    Google Scholar 

  • Ma H, He X (2004) Age and growth of Rhinogobio cylindricus in the mainstream of Yangtze River. Chin J Zool 39:55–59 (in Chinese with English abstract)

    Google Scholar 

  • World Commission on Dams (WCD) (2000) Dams and development: a new framework for decision-making. Earthscan, London

    Google Scholar 

  • Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE 32: Microsoft Windows-based freeware for population genetic analysis. 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by Special Fund for Agro-Scientific Research in the Public Interest (201303056-5) and the Program of Fisheries Genetic Resources Conservation from Ministry of Agriculture of China (2014).

Author information

Authors and Affiliations

  1. College of Animal Science and Technology, Southwest University, Chongqing, 400716, China

    Jiawen Ba & Yun Li

  2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan, 430223, China

    Jiawen Ba, Xiangyang Jia, Dengqiang Wang, Xinbin Duan & Daqing Chen

Authors
  1. Jiawen Ba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiangyang Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dengqiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xinbin Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Daqing Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yun Li.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ba, J., Li, Y., Jia, X. et al. Characterization and cross-species amplification of 19 polymorphic microsatellite loci of Hemibagrus macropterus (Bleeker) in the Yangtze River. Conservation Genet Resour 7, 5–8 (2015). https://doi.org/10.1007/s12686-014-0295-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0295-4

Keywords

Search

Navigation