Abstract
Using next-generation sequencing, we developed and characterized microsatellite primers for Melanoxylon brauna, an important timber species endemic to the Atlantic forest. To characterize the primers, 32 young trees were sampled at one population. Of the 48 primers designed, 43 showed positive amplification and 38 were polymorphic. The combined probability of paternity exclusion was higher than 0.999, and the identity index was lower than 0.001, indicating that the microsatellite primers were suitable for various molecular studies such as paternity testing, gene flow, population structure, and evolution. We believe that the application of this tool in the biological model will contribute to the information on the history of the Atlantic forest, as well as improve strategies and conservation measures for this Brazilian diversity hotspot.
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Acknowledgments
The authors thank to Horlei Ribeiro for technical support. This work was funded by SISBIOTA – CNPq/Fapesb (Fundação de Amparo à Pesquisa do Estado da Bahia) #PES0051/2011 and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) #48703/2012-5. We also thank to CNPq for scholarships to DBB and FAG.
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Borges, D.B., Mariano-Neto, E. & Gaiotto, F.A. Development of microsatellite primers for Melanoxylon brauna (Fabaceae): an endangered and endemic tree from the Brazilian Atlantic Forest. Conservation Genet Resour 7, 65–68 (2015). https://doi.org/10.1007/s12686-014-0288-3
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DOI: https://doi.org/10.1007/s12686-014-0288-3