Skip to main content
SpringerLink
Log in

Development and characterization of genomic microsatellite markers in Rhododendron arboreum

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Population genetics characteristics are the fundamentals of conservation and management practices. Rhododendron arboreum, a key biodiversity component inhabiting Indian Himalayas, suffers from overexploitation and global warming. Using biotin–streptavidin hybridization technique, 41 microsatellite markers were designed from an enriched DNA library to provide a genetic background and an insight into the population structure of the species. With a range of 2–14 alleles amplified from 38 loci, the populations were reported with observed and expected heterozygosity of 0.167–0.933 and 0.422–0.917 respectively. Some of the loci showed significant deviations from Hardy–Weinberg equilibrium and overall no linkage disequilibrium was detected. These markers will support genetic diversity and further genotyping studies in R. arboreum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bhardwaj P, Kumar R, Sharma H, Tewari R, Ahuja PS, Sharma RK (2013) Development and utilization of genomic and genic microsatellite markers in Assam tea (Camellia assamica ssp. assamica) and related Camellia species. Plant Breed 132(6):748–763

    Article  CAS  Google Scholar 

  • Doyle J, Doyle J (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel: population genetic software for teaching and research-update. Bioinformatics 28(19):2537–2539

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Xu J, Grumbine RE, Shrestha A, Eriksson M, Yang X, Wang Y, Wilkes A (2009) Melting Himalayas: cascading effects of climate change on water, biodiversity, and livelihoods. Conserv Biol 23(3):520–530

    Article  CAS  PubMed  Google Scholar 

  • Yeh F, Yang RC, Boyle T (1999) PopGene Version 1.31: Microsoft window based freeware for population genetic analysis. University of Alberta and Centre for International Forestry Research 11–23

Download references

Acknowledgments

The research work present in the manuscript is funded by Department of Science and Technology, Govt. of India. This is CUPB communication no P78/14.

Author information

Authors and Affiliations

  1. Molecular Genetics Laboratory, Centre for Biosciences, Central University of Punjab, Bathinda, 151001, India

    Shruti Choudhary, Sapna Thakur, Ram Gopal Saini & Pankaj Bhardwaj

Authors
  1. Shruti Choudhary
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sapna Thakur
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ram Gopal Saini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pankaj Bhardwaj
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pankaj Bhardwaj.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 157 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Choudhary, S., Thakur, S., Saini, R.G. et al. Development and characterization of genomic microsatellite markers in Rhododendron arboreum . Conservation Genet Resour 6, 937–940 (2014). https://doi.org/10.1007/s12686-014-0246-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0246-0

Keywords

Search

Navigation