Skip to main content
SpringerLink
Log in

Development and characterization of 22 microsatellite loci for the ringed salamander (Ambystoma annulatum) using paired-end Illumina shotgun sequencing

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

We isolated and screened 150 microsatellite loci from the ringed salamander, Ambystoma annulatum, an ambystomatid salamander endemic to the Interior Highlands. Twenty-two polymorphic loci were identified and pooled into two multiplex reactions. These loci will be valuable tools for assessing population genetic structure and connectivity across the landscape, and informing management of this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Castoe TA, Poole AW, de Koning APJ, Jones KL, Tomback DF, Oyler-McCance SJ, Fike JA, Lance SL, Streicher JW, Smith EN, Pollock DD (2012) Rapid microsatellite identification from Illumina paired-end genomic sequencing in two birds and a snake. PLoS ONE 7:e30953

    Article  PubMed  CAS  Google Scholar 

  • Gamble LR, McGarigal K, Compton BW (2007) Fidelity and dispersal in the pond-breeding amphibian, Ambystoma opacum: implications for spatio-temporal population dynamics and conservation. Biol Conserv 139:247–257

    Article  Google Scholar 

  • Greenwald KR, Purrenhage JL, Savage WK (2009) Landcover predicts isolation in Ambystoma salamanders across region and species. Biol Conserv 142:2493–2500

    Article  Google Scholar 

  • Holleley CE, Geerts PG (2009) Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR. Biotechniques 46:511–517

    Article  PubMed  CAS  Google Scholar 

  • Meirmans PG, Van Tienderen PH (2004) GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4:792–794

    Article  Google Scholar 

  • Nunziata SO, Lance SL, Jones KL, Nerkowski SA, Metcalf AE (2013) Development and characterization of twenty-three microsatellite markers for the freshwater minnow Santa Ana speckled dace (Rhinichthys osculus spp., Cyprinidae) using paired-end Illumina shotgun sequencing. Conserv Genet Resour 5:145–148

    Article  Google Scholar 

  • Peterman WE, Connette GM, Spatola BN, Eggert LS, Semlitsch RD (2012) Identification of polymorphic loci in Ambystoma annulatum and review of cross-species microsatellite use in the genus Ambystoma. Copeia 2012:570–577

    Article  Google Scholar 

  • Petranka JW (1998) Salamanders of the United States and Canada. Smithsonian Institution Press, Washington, DC

  • Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Semlitsch RD, Todd BD, Blomquist SM, Calhoun AJK, Gibbons JW, Gibbs JP, Graeter GJ, Harper EB, Hocking DJ, Hunter ML, Patrick DA, Rittenhouse TAG, Rothermel BB (2009) Effects of timber harvest on amphibian populations: understanding mechanisms from forest experiments. Bioscience 59:853–862

    Article  Google Scholar 

Download references

Acknowledgments

We thank S. Lance (University of Georgia) for Illumina sequencing and processing of reads. This research was funded by the DoD Strategic Environmental Research and Development Program (RC2155).

Author information

Authors and Affiliations

  1. Division of Biological Sciences, University of Missouri-Columbia, 110 Tucker Hall, Columbia, MO, 65211, USA

    William E. Peterman, Luke R. Pauley, Emily R. Brocato, Elsa C. Stuart, Raymond D. Semlitsch & Lori S. Eggert

Authors
  1. William E. Peterman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Luke R. Pauley
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Emily R. Brocato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Elsa C. Stuart
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Raymond D. Semlitsch
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lori S. Eggert
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to William E. Peterman.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Peterman, W.E., Pauley, L.R., Brocato, E.R. et al. Development and characterization of 22 microsatellite loci for the ringed salamander (Ambystoma annulatum) using paired-end Illumina shotgun sequencing. Conservation Genet Resour 5, 993–995 (2013). https://doi.org/10.1007/s12686-013-9951-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-013-9951-3

Keywords

Search

Navigation