Technical Note

Conservation Genetics Resources

, Volume 5, Issue 4, pp 989-991

Development and characterization of 18 microsatellite loci for the spotted salamander (Ambystoma maculatum) using paired-end Illumina shotgun sequencing

  • William E. PetermanAffiliated withDivision of Biological Sciences, University of Missouri-Columbia Email author 
  • , Emily R. BrocatoAffiliated withDivision of Biological Sciences, University of Missouri-Columbia
  • , Luke R. PauleyAffiliated withDivision of Biological Sciences, University of Missouri-Columbia
  • , Elsa C. StuartAffiliated withDivision of Biological Sciences, University of Missouri-Columbia
  • , Raymond D. SemlitschAffiliated withDivision of Biological Sciences, University of Missouri-Columbia
  • , Lori S. EggertAffiliated withDivision of Biological Sciences, University of Missouri-Columbia

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Abstract

We isolated and screened 68 microsatellite loci from the spotted salamander, Ambystoma maculatum, in Missouri, USA. Eighteen polymorphic loci were identified and pooled into two multiplex reactions. These loci are a needed addition to the known loci for A. maculatum, which were developed in a different geographic region and are unsuitable for population or landscape genetic analyses in Missouri. To effectively protect and manage this species, regionally-relevant studies of population genetic structure and connectivity are necessary.

Keywords

Ambystoma Population genetics Landscape genetics Multiplex PCR PAL_Finder