Conservation Genetics Resources

, Volume 5, Issue 4, pp 989–991

Development and characterization of 18 microsatellite loci for the spotted salamander (Ambystoma maculatum) using paired-end Illumina shotgun sequencing

Authors

    • Division of Biological SciencesUniversity of Missouri-Columbia
  • Emily R. Brocato
    • Division of Biological SciencesUniversity of Missouri-Columbia
  • Luke R. Pauley
    • Division of Biological SciencesUniversity of Missouri-Columbia
  • Elsa C. Stuart
    • Division of Biological SciencesUniversity of Missouri-Columbia
  • Raymond D. Semlitsch
    • Division of Biological SciencesUniversity of Missouri-Columbia
  • Lori S. Eggert
    • Division of Biological SciencesUniversity of Missouri-Columbia
Technical Note

DOI: 10.1007/s12686-013-9950-4

Cite this article as:
Peterman, W.E., Brocato, E.R., Pauley, L.R. et al. Conservation Genet Resour (2013) 5: 989. doi:10.1007/s12686-013-9950-4

Abstract

We isolated and screened 68 microsatellite loci from the spotted salamander, Ambystoma maculatum, in Missouri, USA. Eighteen polymorphic loci were identified and pooled into two multiplex reactions. These loci are a needed addition to the known loci for A. maculatum, which were developed in a different geographic region and are unsuitable for population or landscape genetic analyses in Missouri. To effectively protect and manage this species, regionally-relevant studies of population genetic structure and connectivity are necessary.

Keywords

AmbystomaPopulation geneticsLandscape geneticsMultiplex PCRPAL_Finder

Copyright information

© Springer Science+Business Media Dordrecht 2013