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Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads

  • Microsatellite Letters
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Abstract

We characterized 22 novel microsatellite loci in the two-spotted octopus Octopus bimaculatus using 454 pyrosequencing reads. All loci were polymorphic and will be used in studies of marine connectivity aimed at increasing sustainability of the resource. The mean number alleles per locus was 13.09 (range 7–19) and observed heterozygosities ranged from 0.50 to 1.00. Four loci pairs were linked and three deviated from Hardy–Weinberg equilibrium. Eighteen and 12 loci were polymorphic in Octopus bimaculoides and Octopus hubbsorum, respectively.

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Acknowledgments

We thank staff from Pronatura Noroeste A. C., Comunidad y Biodiversidad A. C. and the Intercultural Center for the Study of Deserts and Oceans (CEDO A. C.), Funding was provided by The David and Lucile Packard Foundation via the PANGAS project (http://pangas.arizona.edu), the Western Society of Malacologists, and the SIP projects: 20120971, 20121594, 20130059, 20130089, CONACyT 108230. JFDC receive scholarships from CONACyT and PIFI-IPN and the results here presented are part of his Ph.D dissertation.

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Authors and Affiliations

  1. Instituto Politécnico Nacional CICIMAR, Av. Instituto Politécnico Nacional s/n., Col. Playa Palo de Santa Rita, 23096, La Paz, B.C.S., Mexico

    J. F. Domínguez-Contreras, B. P. Ceballos-Vázquez & M. Arellano-Martínez

  2. Conservation Genetics Laboratory, School of Natural Resources and Environment, BSE-317, University of Arizona, 1311 E 4th Street, Tucson, AZ, 85721, USA

    A. Munguía-Vega

  3. Comunidad y Biodiversidad A.C., Isla del Peruano 215, Lomas de Miramar, 85448, Guaymas, Sonora, Mexico

    A. Munguía-Vega

  4. Conservation Genetics Laboratory, Arizona Cooperative Fish and Wildlife Research Unit, School of Natural Resources and Environment, U.S. Geological Survey, BSE-317, University of Arizona, Tucson, AZ, 85721, USA

    M. Culver

Authors
  1. J. F. Domínguez-Contreras
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  2. A. Munguía-Vega
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  3. B. P. Ceballos-Vázquez
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  4. M. Arellano-Martínez
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  5. M. Culver
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Correspondence to J. F. Domínguez-Contreras.

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Domínguez-Contreras, J.F., Munguía-Vega, A., Ceballos-Vázquez, B.P. et al. Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads. Conservation Genet Resour 6, 465–468 (2014). https://doi.org/10.1007/s12686-013-0128-x

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  • DOI: https://doi.org/10.1007/s12686-013-0128-x

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