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PCR-based Y chromosome marker for discriminating between golden jackal (Canis aureus) and domestic dog (Canis lupus familiaris) paternal ancestry

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Abstract

Numerous reports of hybridization between wild Canis species and domestic dog suggest that hybridization between golden jackal and dog is likely. Here we present an assay of a Y chromosome marker for discriminating between golden jackal (Canis aureus) and dog (Canis lupus familiaris) paternal ancestry. Taking advantage of an insertion found in a dog Zfy intron haplotype but not in a golden jackal haplotype, we developed a three-primer PCR system in which species are differentiated based on the number of amplicons. Two amplicons are produced from domestic dog DNA template, while one amplicon is produced from golden jackal DNA template. Both amplicons can be analyzed in a single agarose gel electrophoresis run, while the longer amplicon also serves as an internal control. The method was validated using 27 golden jackal and 25 dog samples; all samples showed the expected amplicons. This simple and cost-effective method should prove useful for determining the direction of golden jackal–dog hybridization.

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References

  • Arnold J, Humer A, Heltai H, Murariu D, Spassov N, Hacklander K (2012) Current status and distribution of golden jackal Canis aureus in Europe. Mammal Rev 42:1–11

    Article  Google Scholar 

  • Fabbri E, Caniglia R, Galov A, Arbanasić H, Lapini L, Bošković I, Florijančić T, Vlasseva A, Ahmed A, Mirchev RL, Randi E (2013) Genetic structure and expansion of golden jackals (Canis aureus) in the north-western distribution range (Croatia and eastern Italian Alps). Conerv Genet. doi:10.1007/s10592-013-0530-7

    Google Scholar 

  • Godinho R, Llaneza L, Blanco JC, Lopes S, Álvares F, García EJ, Palacios V, Cortés Y, Talegón J, Ferrand N (2011) Genetic evidence for multiple events of hybridization between wolves and domestic dogs in the Iberian Peninsula. Mol Ecol 20:5154–5166

    Article  PubMed  Google Scholar 

  • Gomerčić T, Sindičić M, Florijančić T, Bošković I, Huber Đ, Galov A (2013) Differentiating between Y chromosome sequences in Croatian canids. Vet Arhiv 83:571–579

    Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/97/NT. Nucleic Acids Symp Ser 41:95–98

  • Oliveira R, Godinho R, Randi E, Alves PC (2008) Hybridization versus conservation: are domestic cats threatening the genetic integrity of wildcats (Felis silvestris silvestris) in Iberian Peninsula? Philos Trans R Soc Lond B Biol Sci 363(1505):2953–2961

    Article  PubMed Central  PubMed  Google Scholar 

  • Randi E, Lucchini V (2002) Detecting rare introgression of domestic dog genes into wild wolf (Canis lupus) populations by Bayesian admixture analyses of microsatellite variation. Conserv Genet 3:31–45

    CAS  Google Scholar 

  • Rutledge LY, Garroway CJ, Loveless KM, Patterson BR (2010) Genetic differentiation of eastern wolves in Algonquin Park despite bridging gene flow between coyotes and grey wolves. Heredity 105(6):520–531. doi:10.1038/hdy.2010.6

    Article  CAS  PubMed  Google Scholar 

  • Shami K (2002) Evaluating the change in distribution of the eastern timber wolf (Canis lycaon) using the Y-chromosome. Masters thesis, McMaster University

  • Shaw CN, Wilson PJ, White BN (2003) A reliable molecular method of gender determination for mammals. J Mammal 84(1):123–128

    Article  Google Scholar 

  • Vilá C, Walker C, Sundqvist AK, Flagstad Ø, Andersone Z, Casulli A, Kojola I, Valdmann H, Halverson J, Ellegren H (2003) Combined use of maternal, paternal and bi-parental genetic markers for the identification of wolf-dog hybrids. Heredity 90:17–24

    Article  PubMed  Google Scholar 

  • Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden T (2012) Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics 13:134

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zachos FE, Cirovic D, Kirschning J, Otto M, Hartl GB, Petersen B, Honnen A-C (2009) Genetic variability, differentiation, and founder effect in golden jackals (Canis aureus) from Serbia as revealed by mitochondrial DNA and nuclear microsatellite loci. Biochem Genet 47:241–250

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, Zagreb, Croatia

    Ana Galov, Haidi Arbanasić & Matea Baburić

  2. Department for Game Biology, Pathology and Breeding, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia

    Magda Sindičić

  3. Biology Department, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia

    Tomislav Gomerčić

  4. Department for Hunting, Fishery and Beekeeping, Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, Trg Svetog Trojstva 3, Osijek, Croatia

    Ivica Bošković & Tihomir Florijančić

Authors
  1. Ana Galov
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  2. Magda Sindičić
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  3. Tomislav Gomerčić
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  4. Haidi Arbanasić
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  5. Matea Baburić
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  6. Ivica Bošković
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  7. Tihomir Florijančić
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Correspondence to Magda Sindičić.

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Galov, A., Sindičić, M., Gomerčić, T. et al. PCR-based Y chromosome marker for discriminating between golden jackal (Canis aureus) and domestic dog (Canis lupus familiaris) paternal ancestry. Conservation Genet Resour 6, 275–277 (2014). https://doi.org/10.1007/s12686-013-0110-7

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  • DOI: https://doi.org/10.1007/s12686-013-0110-7

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