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Tri-, tetra- and pentanucleotide-repeat microsatellite markers for the Schlegel’s black rockfish Sebastes schlegelii: the potential for reconstructing parentages

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Abstract

We developed non-dinucleotide-repeat microsatellite markers for the Schlegel’s black rockfish Sebastes schlegelii. The so-called 454 pyrosequencing was used for the discovery of repeat arrays. Of 30 microsatellite primer sets, 17 sets worked well for the estimation of polymorphisms. Pentanucleotide-type markers showed a lower variability compared with tri- and tetranucleotide-type markers. The results of simulation-based parentage allocation suggest that these markers will be of great use for reconstructing parentages.

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Acknowledgments

We conducted this study supported by a grant for the recovery of agricultural and fishery industries devastated by the Great East Japan Earthquake (the Agriculture, Forestry and Fisheries Research Council of Japan).

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Authors and Affiliations

  1. Research Center for Aquatic Genomics, National Research Institute of Fisheries Science, Fisheries Research Agency of Japan, 2-12-4 Fuku-ura, Kanazawa, Yokohama, Kanagawa, 2368648, Japan

    Motoshige Yasuike & Masashi Sekino

  2. Miyako Branch, Tohoku National Fisheries Research Institute, Fisheries Research Agency of Japan, 4-9-1 Sakiyama, Miyako, Iwate, 0270097, Japan

    Tsutomu Noda & Yuichiro Fujinami

Authors
  1. Motoshige Yasuike
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  2. Tsutomu Noda
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  3. Yuichiro Fujinami
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  4. Masashi Sekino
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Correspondence to Masashi Sekino.

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Yasuike, M., Noda, T., Fujinami, Y. et al. Tri-, tetra- and pentanucleotide-repeat microsatellite markers for the Schlegel’s black rockfish Sebastes schlegelii: the potential for reconstructing parentages. Conservation Genet Resour 5, 577–581 (2013). https://doi.org/10.1007/s12686-012-9856-6

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  • DOI: https://doi.org/10.1007/s12686-012-9856-6

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