Conservation Genetics Resources

, Volume 5, Issue 2, pp 413–416

Isolation and characterisation of new microsatellite markers for the stonefly Brachyptera braueri comparing a traditional approach with high throughput 454 sequencing

Authors

    • Department of River Ecology and Conservation, Conservation Genetics GroupSenckenberg Research Institutes and Natural History Museums
  • Carsten Nowak
    • Department of River Ecology and Conservation, Conservation Genetics GroupSenckenberg Research Institutes and Natural History Museums
    • Biodiversity and Climate Research Centre (BiK-F)
Technical Note

DOI: 10.1007/s12686-012-9816-1

Cite this article as:
Geismar, J. & Nowak, C. Conservation Genet Resour (2013) 5: 413. doi:10.1007/s12686-012-9816-1

Abstract

We developed thirteen microsatellite markers for the stonefly Brachytpera braueri, a rare aquatic insect. We compared a “traditional” approach yielding an enriched library by cloning of positive host cells, hybridising and Sanger sequencing of target fragments with a 454 next generation sequencing approach. From a total of 881 fragments containing a repeat motif thirteen polymorphic loci were developed and tested for two populations. Number of alleles ranged from 4 to 20 and values of heterozygosity varied from 0.17 to 0.91 (HO) and 0.26 to 0.91 (HE). The markers are essential to investigate the colonisation potential of freshwater insects.

Keywords

Short tandem repeats Parallel pyrosequencing Aquatic insects Dispersal

Copyright information

© Springer Science+Business Media Dordrecht 2012