Technical Note

Conservation Genetics Resources

, Volume 5, Issue 2, pp 413-416

Isolation and characterisation of new microsatellite markers for the stonefly Brachyptera braueri comparing a traditional approach with high throughput 454 sequencing

  • Jutta GeismarAffiliated withDepartment of River Ecology and Conservation, Conservation Genetics Group, Senckenberg Research Institutes and Natural History Museums Email author 
  • , Carsten NowakAffiliated withDepartment of River Ecology and Conservation, Conservation Genetics Group, Senckenberg Research Institutes and Natural History MuseumsBiodiversity and Climate Research Centre (BiK-F)

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Abstract

We developed thirteen microsatellite markers for the stonefly Brachytpera braueri, a rare aquatic insect. We compared a “traditional” approach yielding an enriched library by cloning of positive host cells, hybridising and Sanger sequencing of target fragments with a 454 next generation sequencing approach. From a total of 881 fragments containing a repeat motif thirteen polymorphic loci were developed and tested for two populations. Number of alleles ranged from 4 to 20 and values of heterozygosity varied from 0.17 to 0.91 (HO) and 0.26 to 0.91 (HE). The markers are essential to investigate the colonisation potential of freshwater insects.

Keywords

Short tandem repeats Parallel pyrosequencing Aquatic insects Dispersal