Abstract
Terrestrial salamanders of the genus Plethodon are closely tied to abiotic environmental habitat features and are particularly sensitive to land use and habitat change. To better understand the effects of land use and habitat fragmentation on population genetic characteristics, we screened 137 primers developed from 454 sequence libraries, and developed 27 microsatellite primers for the western slimy salamander, Plethodon albagula. Twenty-two of these primers cross-amplified in P. shermani. These new loci will be invaluable tools to study habitat variables affecting gene flow, population genetic structure, and genetic diversity across heterogeneous landscapes.
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References
Benson G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27:573–580
Collins JT, Taggart T (2009) Standard common and current scientific names for North America amphibians, turtles, reptiles, and crocodilians, 6th edn. http://www.cnah.org/pdf_files/1246.pdf. Accessed 17 Aug 2012
Conant R, Collins JT (1998) Peterson field guides: reptiles and amphibians of Eastern/Central North America. Houghton Mifflin Company
Cushman SA (2006) Effects of habitat loss and fragmentation on amphibians: a review and prospectus. Biol Conserv 128:231–240
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94. doi:10.1111/j.1471-8286.2007.01884.x
Holleley CE, Geerts PG (2009) Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR. Biotechniques 46:511–517
Lance S, Light J, Jones K, Hagen C, Hafner J (2010) Isolation and characterization of 17 polymorphic microsatellite loci in the kangaroo mouse, genus Microdipodops; (Rodentia: Heteromyidae). Conserv Genet Resour 2:139–141
Marsh DM, Page RB, Hanlon TJ, Corritone R, Little EC, Seifert DE, Cabe PR (2008) Effects of roads on patterns of genetic differentiation in red-backed salamanders, Plethodon cinereus. Conserv Genet 9:603–613
Merriman PG, VanTienderen PH (2004) GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4:792–794
Noël S, Ouellet M, Galois P, Lapointe FJ (2007) Impact of urban fragmentation on the genetic structure of the eastern red-backed salamander. Conserv Genet 8:599–606
Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. University of Dublin, Dublin
Petranka JW (1998) Salamanders of the United States and Canada. Smithsonian Institution Press, Washington
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa
Storfer A, Murphy MA, Spear SF, Holderegger R, Waits LP (2010) Landscape genetics: where are we now? Mol Ecol 19:3496–3514
Stuart NS, Chanson JS, Cox NA, Young BE, Rodrigues ASL, Fischman DL, Waller RW (2004) Status and trends of amphibian declines and extinctions worldwide. Science 206:1783–1786
Acknowledgments
We thank C. Cramer (ASU) for sharing 454 sequences for P. albagula, and funding from the University of Missouri Research Board (CB000402), the DoD Strategic Environmental Research and Development Program (RC2155), the Arkansas Biosciences Institute (research component of Arkansas Tobacco Settlement Proceeds Act of 2000), National Science Foundation (DEB-0949590 to KHK), and S.E. Trauth for Arkansas field site access.
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Spatola, B.N., Peterman, W.E., Stephens, N.T. et al. Development of microsatellite loci for the western slimy salamander (Plethodon albagula) using 454 sequencing. Conservation Genet Resour 5, 267–270 (2013). https://doi.org/10.1007/s12686-012-9784-5
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DOI: https://doi.org/10.1007/s12686-012-9784-5