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Development and characterization of eight polymorphic tetra-nucleotide microsatellite markers for the threatened leopard darter (Percina pantherina)

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Abstract

We describe eight tetra-nucleotide microsatellite markers for the leopard darter (Percina pantherina), a federally threatened percid fish endemic to Oklahoma and Arkansas. We tested these markers on 42 individuals from two localities and provide summary statistics on population variability. Eight loci yielded 2–12 alleles per locus. These markers contribute to the availability of markers for programs aimed at monitoring and managing the genetic resources of P. pantherina and related taxa.

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Acknowledgments

We thank D. Fenner, K. Collins, R. Bastarache, B. Bristow and R. Standage for generous assistance in collecting specimens. Collection and permitting for P. pantherina was supervised by the United States Fish and Wildlife Service. We also thank J. Lack for technical advice and assistance in the laboratory. The project was administered by the Oklahoma Cooperative Fish and Wildlife Research Unit at Oklahoma State University. Funding was provided by the United States Forest Service.

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  1. Zoology Department, Oklahoma State University, 501 Life Science West, Stillwater, OK, 74078, USA

    Michael R. Schwemm & Anthony A. Echelle

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  1. Michael R. Schwemm
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  2. Anthony A. Echelle
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Correspondence to Michael R. Schwemm.

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Schwemm, M.R., Echelle, A.A. Development and characterization of eight polymorphic tetra-nucleotide microsatellite markers for the threatened leopard darter (Percina pantherina). Conservation Genet Resour 5, 73–75 (2013). https://doi.org/10.1007/s12686-012-9736-0

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  • DOI: https://doi.org/10.1007/s12686-012-9736-0

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