Abstract
The genus Sclerocactus (Cactaceae) consists of 15 species, which have a confused taxonomic history due to morphological similarities and distribution overlap. Habitat loss and hybridization are of concern and have established cause for genetic investigations to further understand the genus and develop conservation strategies. Thirteen variable microsatellite loci were identified using S. glaucus and S. parviflorus and were tested in three additional species: S. wetlandicus, S. brevispinus and S. cloverii. The mean number of alleles per locus ranged from 2 to 11, with an average of 6.6. The observed and expected mean heterozygosity ranged from 0.00 to 0.90 and 0.26 to 0.90, respectively. These loci will aid in determining levels of hybridization, diversity and taxonomy of Sclerocactus.
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Acknowledgments
This research was funded by the United States Fish and Wildlife Service. Floral tissue was collected by Denver Botanic Gardens and laboratory research was conducted at the University of Northern Colorado.
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Schwabe, A.L., Hubbard, A.R., Neale, J.R. et al. Microsatellite loci development for rare Colorado Sclerocactus (Cactaceae). Conservation Genet Resour 5, 69–72 (2013). https://doi.org/10.1007/s12686-012-9735-1
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DOI: https://doi.org/10.1007/s12686-012-9735-1