Abstract
Here we present 55 new microsatellite markers isolated from the pink-footed goose (Anser brachyrhynchus), using next generation sequencing. Of these, 44 markers were found to be polymorphic, and the number of alleles ranged from two to ten with a mean of 4.23 alleles per locus. Mean observed heterozygosity was 0.44, and 33 polymorphic markers were in Hardy–Weinberg equilibrium in our sample population. These newly developed molecular markers could prove to be a useful tool for further studies of pink-footed goose populations.
References
IPCC (2007) Climate change 2007: synthesis report. In: Core Writing Team, Pachauri RK, Reisinger A (eds) Contribution of working groups I, II and III to the 4th assessment report of the intergovernmental panel on climate change. IPCC, Geneva
Knudsen E, Lindén A, Both C, Jonzén N, Pulido F, Saino N, Sutherland WJ, Bach LA et al (2011) Challenging claims in the study of migratory birds and climate change. Biol Rev (in press)
Madsen J, Cracknell, G, Fox AD (eds) (1999) Goose populations of the Western Palearctic. A review of status and distribution. Wetlands International Publication No. 48. Wetlands International, Wageningen, The Netherlands. National Environmental Research Institute, Rønde, 344 pp
Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404
Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. University of Dublin, Dublin
Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Res 8:103–106
Ruokonen M, Aarvak T, Madsen J (2005) Colonization history of the high-arctic pink-footed goose Anser brachyrhynchus. Mol Ecol 14:171–178
Taggart JB, Hynes RA, Prodöuhl PA, Ferguson A (1992) A simplified protocol for routine total DNA isolation from salmonid fishes. J Fish Biol 40:963–965
Acknowledgments
We thank Minna Ruokonen (University of Oulu) for extracting the DNA and Marika Karjalainen for help in the laboratory. Permits for catching the birds and DNA sampling were issued from the Governor of Svalbard and the Copenhagen Bird Ringing Centre to JM. This study was founded by a grant from the Academy of Finland to PG (grant no. 1123565).
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Noreikiene, K., Teacher, A.G.F., Madsen, J. et al. Isolation and characterization of 55 novel microsatellite markers for the pink-footed goose (Anser brachyrhynchus). Conservation Genet Resour 4, 423–428 (2012). https://doi.org/10.1007/s12686-011-9565-6
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DOI: https://doi.org/10.1007/s12686-011-9565-6