Abstract
Thirteen new polymorphic microsatellite loci were developed and tested in 57 individuals from three locations along the Paraná River. All loci were polymorphic, with 2–22 alleles per locus. Cross-species amplifications were successful for all loci in four other Prochilodus species, suggesting that these microsatellites will be useful for investigation of genetic variation and structure in Prochilodus populations.
References
Agostinho AA, Gomes LC, Suzuki AI, Ferreira Julio H Jr (2003) Migratory fishes of the Upper Paraná River Basin, Brazil. In: Carolsfeld J, Harvey B, Ross CA, Baer A (eds) Migratory fishes of South America: biology fisheries and conservation status. International Development Research Center/The World Bank, Ottawa, pp 99–156
Barbosa ACDR, Correa TC, Galzerani F, Galetti PM Jr (2006) Thirteen polymorphic microsatellite loci in the Neotropical fish Prochilodus argenteus (Characiformes, Prochilodontidae). Mol Ecol Notes 6:936
Bowen SH (1983) Detritivory in Neotropical fish communities. Environ Biol Fish 9:137
Castro RMC, Vari RP (2004) Detritivores of the South American fish family Prochilodontidae (Teleostei: OStariophysi: Characiformes): a phylogenetic and revisionary study. Smithson Contribut Zool 622:1
Espinach Ros A, Sánchez RP (2007) Proyecto Evaluación del Recurso Sábalo en el Paraná. Informe de los resultados de la primera etapa (2005–2006) y medidas de manejo recomendadas. In: Secretaría de Agricultura, Ganaderia, Pesca y Alimentos (ed) Serie Pesca y Acuicultura: Estudios e Investigaciones Aplicadas, Buenos Aires, pp 1–80
Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47
Flecker AS (1996) Ecosystem engineering by a dominant detritivore in a diverse tropical stream. Ecology 77:1845
Hatanaka T, Henrique-Silva F, Galetti PM Jr (2006) Population substructuring in a migratory freshwater fish Prochilodus argenteus (Characiformes, Prochilodontidae) from the São Francisco River. Genetica 126:153
Kandpal RP, Kandpal G, Weissman SM (2004) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers. In: Proceedings of the national academy of sciences, USA, vol 91, p 88
Kawakami de Resende E (2003) Migratory fishes of the Paraguay-Paraná Basin, excluding the Upper Paraná Basin. In: Carolsfeld J, Harvey B, Ross CA, Baer A (eds) Migratory fishes of South America: biology fisheries and conservation status. International Development Research Center/The World Bank, Ottawa, pp 99–156
Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365
Valenciennes A (1834–42) Poissons [plates]. In: d'Orbigny A (eds) Voyage dans l’Amérique méridionale. Voyage dans l’Amérique méridionale. Pls. 1–16
Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535
Yazbeck GM, Kalapothakis E (2007) Isolation and characterization of microsatellite DNA in the piracema fish Prochilodus lineatus (Characiformes). Genet Mol Res 6:1026
Zayas MA, Cordiviola E (2007) The conservation state of characidae fish (Pisces: Characiformes) in an area of the Plata Basin, Argentina. Gayana 71:178
Acknowledgments
A postdoctoral fellowship to ECR from CONICET (Argentina) and NSF grants DEB-0443470 to GO and INT-9117104 to M.A. Bell and A. Meyer supported this work. Thanks to Kerry Mullaney for lab support.
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Rueda, E.C., Sommer, J., Scarabotti, P. et al. Isolation and characterization of polymorphic microsatellite loci in the migratory freshwater fish Prochilodus lineatus (Characiformes: Prochilodontidae). Conservation Genet Resour 3, 681–684 (2011). https://doi.org/10.1007/s12686-011-9432-5
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DOI: https://doi.org/10.1007/s12686-011-9432-5