Abstract
Thirteen tetranucleotide microsatellite loci were isolated from tetranucleotide microsatellite enriched libraries for the Tokay gecko (gekko gecko). The species is endemic to Southeast Asia, known to be a habitat specialist, and exposed to severe hunting pressures in the mountainous areas of Southeast Asia. These highly polymorphic markers (6–25 alleles) will facilitate the population genetic analyses of this species. In particular, they will enable estimates of gene flow between the two types of the Tokay gecko: the black-spotted Tokay gecko and the red-spotted Tokay gecko-a critical element in assessing classification between the two.
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References
Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gel. Anal Biochem 195:80–83
Ellegren H (2004) Microsatellites: simple sequences with complex evolution. Genetics 5:435–445
Hartl DL, Clark AG (1997) Principle of population genetics. Sinauer Associates, Sunderland, MA
Li HH, Tang ZJ, Yu TL, Chen M, Huang CM (1996) Resoures and protection of Gekko gecko in Guangxi, J. Guangxi Normal Univ 14:62–66
Liu ZQ, Wang YQ, Zhou KY, Han DM (2000) Two haplotypes of Gekko gecko on the mitochondrial 12S rRNA gene sequences. J Anhui Normal Univ 23:339–350
Peng QK, Wang GC, Yang D, Yue BS, Li L, Zou FD (2010) Genetic variability of the tokay gecko based on microsatellite analysis. Biochem Syst Eco 38:23–28
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Sambrook J, Russell DW (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 2nd edn, CSHL Press, New York
Yuan JQ, Li L (2008) Herbal textual of black-spot and red-spot Gekko gecko. J Chin Med Materials 9:1437–1439
Zhang QQ, Tang YZ, Huang YC, Zeng FH (1997) The preliminary study on the geographic variability of the toke gecko (Gekko gecko). Chin J Zool 32:44–46
Zou FD, Yue BS, Xu L, Zhang YZ (2005) Isolation and characterization of microsatellite loci from forest musk deer (Moschus berezovskii). Zool Sci 22:593–598
Acknowledgments
This research was supported by the Program for New Century Excellent Talents in University and by the Science and Technology Supporting Program of National Eleventh Five-Year Project (2006BAI06A12-03).
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Quekun Peng, Gaochao Wang are contributed equally to this work.
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Peng, Q., Wang, G., Tan, S. et al. Isolation and characterization of thirteen polymorphic tetranucleotide microsatellite markers in the Tokay gecko (Gekko Gecko). Conservation Genet Resour 3, 617–619 (2011). https://doi.org/10.1007/s12686-011-9417-4
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DOI: https://doi.org/10.1007/s12686-011-9417-4