Skip to main content
SpringerLink
Log in

Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Ifremeria nautilei is deep-sea provannid gastropod endemic to hydrothermal vents at southwest Pacific back-arc spreading centers. Twelve, selectively neutral and unlinked polymorphic microsatellite loci were developed for this species. Three loci deviated significantly from Hardy–Weinberg expectations. Average observed heterozygosity ranged from 0.719 to 0.906 (mean H O  = 0.547, SD = 0.206). Three of the 12 loci cross-amplified in two species of Alviniconcha (Provannidae) that co-occur with I. nautilei at Pacific vent habitats. Microsatellites developed for I. nautilei are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Antao T, Lopes A, Lopes RJ, Beja-Pereira A, Luikart G (2008) LOSITAN: a workbench to detect molecular adaptation based on a Fst-outlier method. BMC Bioinformatics 9:323

    Article  PubMed  Google Scholar 

  • Bouchet P, Warén A (1991) Ifremeria nautilei, a new gastropod from hydrothermal vents, probably associated with symbiotic bacteria. C R Acad Sci III 312(10):495–501

    Google Scholar 

  • Carney SL, Formica MI, Divatia H, Nelson K, Fisher CR, Schaeffer SW (2006) Population structure of the mussel “Bathymodiolus” childressi from Gulf of Mexico hydrocarbon seeps. Deep Sea Res I 53(6):1061–1072

    Article  Google Scholar 

  • Daguin C, Jollivet D (2005) Development and cross-amplification of nine polymorphic microsatellite markers in the deep-sea hydrothermal vent polychaete Branchipolynoe seepensis. Mol Ecol Notes 5(4):780–783

    Article  CAS  Google Scholar 

  • Desbruyères D, Hashimoto J, Fabri MC (2006) Composition and biogeography of hydrothermal vent communities in western Pacific back-arc basins. Geophys Monogr 166:215–234

    Google Scholar 

  • Fusaro AJ, Baco AR, Gerlach G, Shank TM (2008) Development and characterization of 12 microsatellite markers from the deep-sea hydrothermal vent siboglinid Riftia pachyptila. Mol Ecol Res 8(1):132–134

    Article  CAS  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. In: Zimmer EA, Roalson EH (eds) Methods in enzymology 395, molecular evolution: producing the biochemical data, Part B. Academic Press, San Diego, pp 202–222

    Chapter  Google Scholar 

  • Halfar J, Fujita RM (2002) Precautionary management of deep-sea mining. Mar Policy 26(2):103–106

    Article  Google Scholar 

  • Hurtado LA, Lutz RA, Vrijenhoek RC (2004) Distinct patterns of genetic differentiation among annelids of eastern Pacific hydrothermal vents. Mol Ecol 13(9):2603–2615

    Article  CAS  PubMed  Google Scholar 

  • Kojima S, Segawa R, Fijiwara Y, Fujikura K, Ohta S, Hashimoto J (2001) Phylogeny of hydrothermal-vent-endemic gastropods Alviniconcha spp. from the Western Pacific revealed by mitochondrial DNA sequences. Biol Bull 200(3):298–304

    Article  CAS  PubMed  Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43(1):223–225

    Article  Google Scholar 

  • Rona PA (2003) Resources of the sea floor. Science 299:673–674

    Article  CAS  PubMed  Google Scholar 

  • Rousset F (2009) genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnol 18:233–234

    Article  CAS  Google Scholar 

  • Shank TM, Halanych KM (2007) Toward a mechanistic understanding of larval dispersal: insights from genomic fingerprinting of the deep-sea hydrothermal vent tubeworm Riftia pachyptila. Mar Ecol 28(1):25–35

    Article  CAS  Google Scholar 

  • Suzuki Y, Kojima S, Watanabe H, Suzuki M, Tsuchida S, Nunoura T, Hirayama H, Takai K, Nealson K, Horikoshi K (2006) Single host and symbiont lineages of hydrothermal-vent gastropods Ifremeria nautilei (Provannidae): biogeography and evolution. Mar Ecol Prog Ser 315:167–175

    Article  CAS  Google Scholar 

  • Thurston MI, Field D (2005) Msatfinder: detection and characterisation of microsatellites. Distributed by the authors at http://www.genomics.ceh.ac.uk/msatfinder/. CEH Oxford, Mansfield Road, Oxford OX1 3SR

  • Urakawa H, Dubilier N, Fujiwara Y, Cunningham DE, Kojima S, Stahl DA (2005) Hydrothermal vent gastropods from the same family (Provannidae) harbour ε- and γ-proteobacterial endosymbionts. Environ Microbiol 7(5):750–754

    Article  CAS  PubMed  Google Scholar 

  • van Oosterhout C, Hutchinson W, Willds D, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by a research contract from Nautilus Minerals Niugini Limited to CLVD, JC, and TS and by Duke University. JC acknowledges support from the Beaufort Marine Research Award in Fish Population Genetics funded by the Irish Government under the Sea Change Programme. Samples used in this study were collected on behalf of the people of Papua New Guinea.

Author information

Authors and Affiliations

  1. Marine Conservation Molecular Facility, Marine Laboratory, Nicholas School of the Environment, Duke University, 135 Duke Marine Lab Road, Beaufort, NC, 28516, USA

    Andrew David Thaler, Kevin Zelnio, Rebecca Jones, Jens Carlsson, Cindy Lee Van Dover & Thomas F. Schultz

  2. Department of Zoology, Ecology and Plant Science, University College Cork, Distillery Fields, North Mall, Cork, Ireland

    Jens Carlsson

Authors
  1. Andrew David Thaler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kevin Zelnio
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rebecca Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jens Carlsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cindy Lee Van Dover
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Thomas F. Schultz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andrew David Thaler.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thaler, A.D., Zelnio, K., Jones, R. et al. Characterization of 12 polymorphic microsatellite loci in Ifremeria nautilei, a chemoautotrophic gastropod from deep-sea hydrothermal vents. Conservation Genet Resour 2, 101–103 (2010). https://doi.org/10.1007/s12686-010-9174-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-010-9174-9

Keywords

Search

Navigation