Skip to main content
SpringerLink
Log in

Development of 13 polymorphic microsatellite loci for the Greenland Halibut (Reinhardtius hippoglossoides)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

We report the development of 13 variable microsatellite loci for the Greenland Halibut (Reinhardtius hippoglossoides). In a sample of 72 individuals from Western Greenland we report heterozygosities ranging from 0.15 to 1.0 (observed) and 0.14 to 0.97 (expected). One locus was found to have a low frequency of null alleles (0.065), all others are in HWE and no evidence of linkage disequilibrium among loci was found.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Boje J (2002) Intermingling and seasonal migrations of Greenland halibut (Reinhardtius hippoglossoides) populations determined from tagging studies. Fish Bull 100:414–422

    Google Scholar 

  • Boje J, Riget F, Køie M (1997) Helminth parasites as biological tags in population studies of Greenland halibut ((Reinhardtius hippoglossoides) (Walbaum)), in the north-west Atlantic. ICES J Mar Sci 54:886–895

    Article  Google Scholar 

  • Bowering WR, Brodie WB (1995) Greenland halibut (Rheinardtius hippoglossoides): a review of the dynamics of its distribution and fisheries off eastern Canada and Greenland. In: Hopper AG (ed) Deep-water fisheries of the North Atlantic slope. Kluwer Academic Publishers, The Netherlands, pp 113–160

    Google Scholar 

  • Coad BW (1995) Encyclopedia of Canadian fishes. Canadian Museum of Nature and Canadian Sportfishing Productions Inc. Key Porter Books, Singapore, 944 pp

  • DFO (2008) Cumberland Sound Greenland Halibut (Turbot) Inshore Fishery. DFO Can. Sci. Advis. Sec. Sci. Advis. Rep. 2008/040

  • DFO (2009) Assessment of the Greenland Halibut Stock in the Gulf of St. Lawrence (4RST) in 2008. DFO Can. Sci. Advis. Sec. Sci. Advis. Rep. 2009/020

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA Loci. Meth Enzymol 395:202–222

    Article  CAS  PubMed  Google Scholar 

  • Goudet J (1995) FSTAT (version 2.93) a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • NAFO (2008) Scientific Council Reports 2008. Northwest Atlantic Fisheries Organization, Dartmouth, Nova Scotia, Canada, 328 pp

  • Oetting WS, Lee HK, Flanders DJ, Weisner GL, Sellers TA, King RA (1995) Linkage analysis with multiplexed short tandem repeat polymorphisms using infrared fluorescence and M13 tailed primers. Genomics 30:450–458

    Article  CAS  PubMed  Google Scholar 

  • Pomilla C, Treble MA, Postma LD, Lindsay MM, Reist JD (2008) Initial genetic evidence of population structure of Greenland halibut (Reinhardtius hippoglossoides) in the Northwest Atlantic. J Northwest Atl Fish Sci 40:1–15

    Article  Google Scholar 

  • Riget F, Boje J, Simonsen V (1992) Analysis of meristic characters and genetic differentiation in Greenland halibut (Reinhardtius hippoglossoides) in the Northwest Atlantic. J Northwest Atl Fish Sci 12:7–14

    Article  Google Scholar 

  • Rousset R (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  • Rozen S, Skaletsky H (2000) PRIMER 3 on the WWW for general users and for biologists programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Towoto, New Jersey, pp 365–386

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  • Templeman W (1970) Vertebral and other meristic characteristics of Greenland halibut, Reinhardtius hippoglossoides, from the Northwest Atlantic. J Fish Res Board Can 27:1549–1562

    Google Scholar 

  • Treble MA, Jørgensen OA (2002) Summary of results for Greenland halibut from trawl surveys conducted in NAFO Subareas 0 and 1 from 61°N to 74°N in 2001. NAFO SCR Doc. 02/60

  • van Oosterhout C, Hutchinson WF, Willis DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • Vis ML, Carr SM, Bowering WR, Davidson S (1997) Greenland halibut (Reinhardtius hippoglossoides) in the North Atlantic are genetically homogeneous. Can J Fish Aquat Sci 54:1813–1821

    Article  Google Scholar 

Download references

Acknowledgements

Fish tissue collections were generously provided by Mikel Casa, Instituto Español de Oceanografía and Ole Jørgensen, DTU-AQUA, Technical University of Denmark. Funding was provided by a grant from the International Governance Strategy and National Centre for Arctic Aquatic Research Excellence programs of Fisheries and Oceans Canada (DFO) to MT and DER as well as by an NSERC Discovery Grant to DER. We thank Abby van der Jagt and Sarah Reid-Holland for some of the laboratory work.

Author information

Authors and Affiliations

  1. Marine Gene Probe Laboratory, Department of Biology, Dalhousie University, Halifax, NS, B3H 4J1, Canada

    Ian G. Paterson & Daniel E. Ruzzante

  2. Department of Fisheries and Oceans Canada, Bedford Institute of Oceanography, 1 Challenger Drive, Dartmouth, NS, B2Y 4A2, Canada

    Dave C. Hardie

  3. Department of Fisheries and Oceans Canada, 501 University Crescent, Winnipeg, MB, R3T 2N6, Canada

    Margaret A. Treble

Authors
  1. Ian G. Paterson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dave C. Hardie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Margaret A. Treble
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Daniel E. Ruzzante
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ian G. Paterson.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Paterson, I.G., Hardie, D.C., Treble, M.A. et al. Development of 13 polymorphic microsatellite loci for the Greenland Halibut (Reinhardtius hippoglossoides). Conservation Genet Resour 2, 97–100 (2010). https://doi.org/10.1007/s12686-010-9173-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-010-9173-x

Keywords

Search

Navigation