Abstract
Our research on slimy sculpins (Cottus cognatus), a benthic, freshwater fish, requires highly polymorphic genetic markers, and microsatellite loci developed for other Cottus species were insufficient for our needs. We therefore developed 13 polymorphic microsatellite loci from C. cognatus libraries enriched for tri- and tetranucleotide repeats. These loci had 2–22 alleles and observed heterozygosities ranging from 0.36 to 0.86 in a sample of 47 individuals from one population. There was no evidence of linkage disequilibrium; however, one locus had a putative null allele. Twelve loci also worked for mottled sculpin (Cottus bairdi) but only eight were polymorphic in a sample of seven individuals.
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Acknowledgments
This work is the result of research sponsored by the Minnesota Sea Grant Research Program supported by the NOAA office of Sea Grant, United States Department of Commerce, under grant No. R/F33-09. The U.S. Government is authorized to reproduce and distribute reprints for government purposes, not withstanding any copyright notation that may appear hereon. This paper is journal reprint No. 565 of the Minnesota Sea Grant College Program.
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Fujishin, L.M., Keith Barker, F., Huff, D.D. et al. Isolation of 13 polymorphic microsatellite loci for slimy sculpin (Cottus cognatus). Conservation Genet Resour 1, 429 (2009). https://doi.org/10.1007/s12686-009-9099-3
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DOI: https://doi.org/10.1007/s12686-009-9099-3