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Recombinant Plasmid DNA Construct Encoding Combination of vegf165 and bmp2 cDNAs Stimulates Osteogenesis and Angiogenesis In Vitro

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Abstract

Though normally bone regenerates well, non-union, delayed union, or defective bone formation can occur. Since impaired healing is often caused by local trophic disorders, standard fracture management is often ineffective. Gene therapy is a promising field for bone engineering. We have created a recombinant plasmid construct encoding the genes for pro-angiogenic vascular endothelial growth factor 165A (VEGF165A) and pro-osteogenic bone morphogenetic protein 2 (BMP2) with kanamycin resistance (pBudKan-VEGF165A-BMP2). This plasmid has the potential for use in treatment of trauma and skeletal system disorders, especially in cases of low trophicity.

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Acknowledgments

The work was performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities. Some of the experiments were conducted using the equipment of Interdisciplinary center for collective use of the Kazan Federal University, Interdisciplinary Center for Analytical Microscopy, and Pharmaceutical Research and Education Center, Kazan (Volga Region) Federal University, Kazan, Russia.

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Authors and Affiliations

  1. Kazan Federal University, Kazan, Russia

    M. N. Zhuravleva, M. R. Khaliullin, R. F. Masgutov, R. V. Deev & A. A. Rizvanov

  2. Republican Clinical Hospital, Kazan, Russia

    R. F. Masgutov

Authors
  1. M. N. Zhuravleva
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  2. M. R. Khaliullin
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  3. R. F. Masgutov
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  4. R. V. Deev
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  5. A. A. Rizvanov
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Correspondence to A. A. Rizvanov.

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The authors declare that they no conflicts of interest.

Electronic Supplementary Material

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Supplementary Material 1

Codon-optimized VEGF165A and BMP2 genes sequences. (PDF 186 kb)

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Zhuravleva, M.N., Khaliullin, M.R., Masgutov, R.F. et al. Recombinant Plasmid DNA Construct Encoding Combination of vegf165 and bmp2 cDNAs Stimulates Osteogenesis and Angiogenesis In Vitro. BioNanoSci. 7, 288–293 (2017). https://doi.org/10.1007/s12668-016-0300-3

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  • DOI: https://doi.org/10.1007/s12668-016-0300-3

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