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2(5H)-Furanone Derivatives as Inhibitors of Staphylococcal Biofilms

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Abstract

The opportunistic bacteria Staphylococcus aureus and Staphylococcus epidermidis often form rigid biofilms on wounds and artificial surfaces and thereby become extremely resistant to antimicrobials. Here, we report the effect of four novel 2(5H)-furanone derivatives on the cell growth and biofilm formation by these microorganisms. Using the differential fluorescence staining of viable and dead cells, we demonstrated that furanones increase the antibacterial efficacy of chloramphenicol against both biofilm-embedded S. aureus and S. epidermidis with F35 being the most efficient compound, probably by increasing the accessibility of cells against antimicrobials. Compounds F6, F8, and F83 inhibited the biofilm formation at concentrations of 2.5–10 μg/ml, although exhibiting high cytotoxicity for human skin fibroblasts with CC50 of 0.5–1.1 μg/ml. F35 demonstrated minimal biofilm inhibition concentration of 10 μg/ml, while its cytotoxicity was ten times lower than that of the other compounds (CC50 13.1 μg/ml), suggesting its chemotype seems a promising starting point for the development of new antibiofilm agents.

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Acknowledgments

This work was supported by the Russian Science Foundation (RSF 15-14-00046) and by the Russian Government Program to support the competitive development of the Kazan Federal University.

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Authors and Affiliations

  1. Institute of Fundamental Medicine and Biology, Kazan Federal University, 18 Kremlyovskaya Street, Kazan, 420008, Russia

    Elena Trizna, Mikhail I. Bogachev & Airat Kayumov

  2. Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, 18 Kremlyovskaya Street, Kazan, 420008, Russia

    Liliya Latypova & Almira Kurbangalieva

  3. Biomedical Engineering Research Center, Saint Petersburg Electrotechnical University, 5 Professor Popov Str., 197376, St. Petersburg, Russia

    Mikhail I. Bogachev

Authors
  1. Elena Trizna
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  2. Liliya Latypova
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  3. Almira Kurbangalieva
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  4. Mikhail I. Bogachev
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  5. Airat Kayumov
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Correspondence to Airat Kayumov.

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Trizna, E., Latypova, L., Kurbangalieva, A. et al. 2(5H)-Furanone Derivatives as Inhibitors of Staphylococcal Biofilms. BioNanoSci. 6, 423–426 (2016). https://doi.org/10.1007/s12668-016-0258-1

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  • DOI: https://doi.org/10.1007/s12668-016-0258-1

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