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In vitro production of Toxocara canis excretory-secretory (TES) antigen

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Abstract

Toxocara canis is a widespread gastrointestinal nematode parasite of dogs and cause Toxocara larva migrans, an important zoonotic disease in humans on ingestion of infective eggs. Toxocarosis is one of the few human parasitic diseases whose serodiagnosis uses a standardized antigen, T. canis excretory secretory antigen (TES). The present study describes collection of T. canis adult worm, collection and embryonation of T. canis eggs, hatching and separation of T. canis larvae, in vitro maintenance of T. canis second stage larvae for production of TES, concentration of culture fluid TES and yield of TES in correlation with various methods cited in literature.

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References

  • Alcantara-Neves NM, dos Santos AB, Mendoca LR, Figuereido CA, Pontes de Carvalho L (2008) An improved method to obtain antigen excreting Toxocara canis larvae. Exp Parasitol 119:349–351

    Article  CAS  PubMed  Google Scholar 

  • Aydenizoz-Ozkayhan M, Yagu BB, Erat S (2008) The investigation of Toxocara canis eggs in coats of different dog breeds as a potential transmission route in human toxocariasis. Vet Parasitol 152:94–100

    Article  CAS  PubMed  Google Scholar 

  • Badley JE, Grieve RB, Bowman DD, Glickman LT, Rockey JH (1987) Analysis of Toxocara canis excretory secretory antigens: physiochemical characterization and antibody detection. Parasitol 73:593–600

    Article  CAS  Google Scholar 

  • Beaver PC, Snyde CH, Carrera GM (1952) Chronic eosinophilia due to visceral larva migrans. Pediatrics 9:7–19

    CAS  PubMed  Google Scholar 

  • Bowman DD, Grieve M, Grieve RB (1987) Circulating excretory antigen levels and specific antibody responses in mice infected with Toxocara canis. Am J Trop Med Hyg 36:75–82

    Article  CAS  PubMed  Google Scholar 

  • Boyce WM, Branstetter BA, Kazacos KR (1988) Comparative analysis of larval excretory-secretory antigens of Bayliascaris procyonis, Toxocara canis and Ascaris suum by western blotting and enzyme immunoassay. Int J Parasitol 18:109–113

    Article  CAS  PubMed  Google Scholar 

  • Colli CM, Rubinsky-Elefant G, Paludo ML, Falavigna DLM, Guilherem EV, Mattia S, Araujo SM, Ferreira EC, Previdelli ITS, Flavigna-Guilherem AL (2010) Serological, clinical and epidemiological evaluation of toxocariasis in urban areas of South Brazil. Rev Inst Med Trop Sao Paulo 52:69–74

    Article  PubMed  Google Scholar 

  • Crowcroft NS, Gillespie SH (1991) Hatching of second stage larvae of Toxocara canis: a rapid method for processing large numbers of worms. J Helminthol 65:311–312

    Article  CAS  PubMed  Google Scholar 

  • de Savigny DH (1975) In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara ES antigen for use in serodiagnostic tests for visceral larva migrans. J Parasitol 61:781–782

    Article  CAS  PubMed  Google Scholar 

  • de Savigny DH, Tizzard IR (1977) Toxocara larva migrans: the use of larval secretory antigens in haemagglutination and soluble antigen fluorescent antibody tests. Trans Roy Soc Trop Med Hyg 71:501–507

    Article  PubMed  Google Scholar 

  • de Savigny DH, Voller A, Woodruff AW (1979) Toxocariasis: serological diagnosis by enzyme linked immmunosorbent assay. J Clin Pathol 32:284–288

    Article  PubMed  PubMed Central  Google Scholar 

  • Girdwood RWA, Smith HV, Bruce RG, Quinn R (1978) Human Toxocara infection in the West of Scotland. Lancet 1:1318

    Article  CAS  PubMed  Google Scholar 

  • Glickman LT, Schantz PM (1981) Epidemiology and pathogenesis of zoonotic toxocariasis. Epidemiol Rev 3:230–250

    Article  CAS  PubMed  Google Scholar 

  • Glickman LT, Grieve RB, Lauria S, Jones DL (1985) Serodiagnosis of ocular toxocariasis: a comparison of two antigens. J Clin Pathol 38:103–107

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gupta AK (1984) A simple method of artificial hatching of Toxocara canis in vitro and preparation of excretory secretory antigen. Curr Sci 53:529–530

    Google Scholar 

  • Iddawela RD, Rajapakse RPVJ, Perera NAND, Agastuma T (2007) Characterization of Toxocara canis species specific excretory secretory antigen and development of a double sandwich ELISA for diagnosis of visceral larva migrans. Korean J Parasitol 1:19–26

    Article  Google Scholar 

  • Kim Y, Huh S, Chung Y (2008) Seroprevalence of toxocariasis among healthy people with eosinophilia. Korean J Parasitol 1:29–32

    Article  Google Scholar 

  • Magnaval JF, Glickman LT, Dorchies PH (1994) La toxocarise, une zoonose helminthique majeure. Rev Med Vet 145:611–627

    Google Scholar 

  • Magnaval JF, Galindo V, Glickman LT, Clanet M (1997) Human Toxocara infection of the central nervous system and neurological disorders: a case control study. Parasitol 115:537–543

    Article  Google Scholar 

  • Magnaval JF, Glickman LT, Dorchies P, Morassin B (2001) Highlights of human toxocariasis. Korean J Parasitol 39:1–11

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Maizels RM, de Savigny DH, Ogilvie BM (1984) Characterization of surface and excretory secretory antigens of Toxocara canis infective larvae. Parasite Immunol 6:23–37

    Article  CAS  PubMed  Google Scholar 

  • Mizgajska H (2001) Eggs of Toxocara spp in the environment and their public health implication. J Helminthol 75:147–151

    CAS  PubMed  Google Scholar 

  • Morales OL, Lopez MC, Nicholls RS, Agudelo C (2002) Identification of Toxocara canis antigens by western blot in experimentally infected rabbits. Rev Inst Med Trop Sao Paulo 44:213–216

    Article  PubMed  Google Scholar 

  • Nagakura K, Tachibana H, Kaneda Y, Kato Y (1989) Toxocariasis possibly caused by ingesting raw chicken. J Infect Dis 160:735–736

    Article  CAS  PubMed  Google Scholar 

  • Nagakura K, Kanno S, Tachibana H, Kaneda Y, Ohkido M, Kondo K, Inoue H (1990) Serologic differentiation between Toxocara canis and Toxocara cati. J Infect Dis 162:1418–1419

    Article  CAS  PubMed  Google Scholar 

  • Oaks JA, Kayes SG (1979) Artificial hatching and culture of Toxocara canis second stage larvae. J Parasitol 65:969–970

    Article  CAS  PubMed  Google Scholar 

  • Ponce-Macotela M, Cabellero AR, Abarca GEP, Gordillo MNM (2011) A simplified method for hatching and isolating Toxocara canis larvae to facilitate excretory-secretory antigen collection in vitro. Vet Parasitol 175:382–385

    Article  PubMed  Google Scholar 

  • Rajapakse RPVJ, Vasanthathilake VWSM, Fernando ST (1992) Collection of eggs and hatching and culturing second stage larvae of Toxocara vitulorum in vitro. J Parasitol 78:1090–1092

    Article  CAS  PubMed  Google Scholar 

  • Roddie G, Stafford P, Holland C, Wolfe A (2008) Contamination of dog hair with eggs of Toxocara canis. Vet Parasitol 152:85–93

    Article  PubMed  Google Scholar 

  • Roldan WH, Espinoza YA (2009) Evaluation of an enzyme linked immunoelectrotransfer blot test for the confirmatory serodiagnosis of human toxocariasis. Mem Inst Oswaldo Cruz 104:411–418

    Article  CAS  PubMed  Google Scholar 

  • Roldan W, Cornejo W, Espinoza Y (2006) Evaluation of the dot enzyme linked immuno sorbent assay in comparison with standard ELISA for the immunodiagnosis of human toxocariasis. Mem Inst Oswaldo Cruz 101:71–74

    Article  CAS  PubMed  Google Scholar 

  • Rubinsky-Elefant G, da Silva-Nunes M, Malafronte RS, Muniz PT, Ferreira MU (2008) Human toxocariasis in rural Brazilian Amazonia: seroprevalence, risk factors and spatial distribution. Am J Trop Med Hyg 79:93–98

    Article  PubMed  Google Scholar 

  • Shobana M (2003) Immunodiagnosis of Toxocara canis infection. M.V.Sc. thesis submitted to the Tamilnadu Veterinary and Animal Science University, Chennai

  • Smith HV (1989) A rapid method for hatching infective eggs of Toxocara canis. Trans Roy Soc Trop Med Hyg 83:215

    Article  CAS  PubMed  Google Scholar 

  • Smith HV, Quinn R, Bruce RG, Girdwood RWA (1980) A paper radioimmunosorbent test (PRIST) for the detection of larva specific antibodies to Toxocara canis in human sera. J Immunol Methods 37:47–55

    Article  CAS  PubMed  Google Scholar 

  • Smith HV, Girdwood RWA, Quinn R, Bruce RG (1982) The development of early serological responses in rabbits infected with Toxocara canis and Toxascaris leonina . Trans R Soc Trop Med Hyg 76:89–94

    Article  CAS  PubMed  Google Scholar 

  • Sturchler D, Weiss N, Gassner M (1990) Transmission of toxocariasis. J Infect Dis 162:571–572

    Article  CAS  PubMed  Google Scholar 

  • Sugane K, Oshima T (1983) Purification and characterization of excretory secretory antigen of Toxocara canis larvae. Immunology 50:113–120

    CAS  PubMed  PubMed Central  Google Scholar 

  • Taira K, Saeed I, Permin A, Kapel CM (2004) Zoonotic risk of Toxocara canis infection through consumption of pig or poultry viscera. Vet Parasitol 121:115–124

    Article  CAS  PubMed  Google Scholar 

  • Taylor M, Keane C, O’ Connor P, Mulvihill E, Holland C (1988) The expanded spectrum of toxocaral disease. Lancet 1:692–695

    Article  CAS  PubMed  Google Scholar 

  • van Knapen F, van Leusden J, Polderman AM, Franchimont JH (1983) Visceral larva migrans: examinations by means of enzyme linked immunosorbent assay of human sera for antibodies to excretory secretory antigens of the second stage larvae of Toxocara canis. Z Parasitenkd 69:113–118

    Article  PubMed  Google Scholar 

  • Vasquez Tsuji O, Barbabosa IM, Zavala JT, Hernandez AR, Torres HP (1997) Vegetables for human consumption as probable source of Toxocara spp. infection in man. Bol Chil Parasitol 52:47–50

    Google Scholar 

  • Watthanakulpanich D, Smith HV, Hobbs G, Walle AJ, Billington D (2008) Application of Toxocara canis excretory secretory antigen and IgG subclass antibodies (IgG 1–4) in serodiagnostic assays of human toxocariasis. Acta Trop 106:90–95

    Article  CAS  PubMed  Google Scholar 

  • Williamson HJE, Allardyce RA, Clemell RS, Hidajat RR (1990) Serum and neutrophils alter the rate of excretory secretory antigen release by Toxocara canis infective larvae in vitro. Parasite Immunol 12:175–187

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors wishes to thank Dr. S.A. ASOKAN, Dean, Madras Veterinary College, Chennai, India for the facilities provided. The work was supported by Indian Council of Agricultural Research, New Delhi, India with financial assistance in the form of Junior Research Fellowship.

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Authors and Affiliations

  1. Department of Veterinary Parasitology, Madras Veterinary College (TANUVAS), Chennai, India

    Divyamol Thomas & S. Abdul Basith

  2. Department of Veterinary Parasitology, Veterinary College and Research Institute (TANUVAS), Orathanadu, India

    N. Jeyathilakan

  3. Department of Animal Biotechnology, Madras Veterinary College (TANUVAS), Chennai, India

    T. M. A. Senthilkumar

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  1. Divyamol Thomas
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  2. N. Jeyathilakan
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  3. S. Abdul Basith
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  4. T. M. A. Senthilkumar
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Correspondence to N. Jeyathilakan.

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Thomas, D., Jeyathilakan, N., Abdul Basith, S. et al. In vitro production of Toxocara canis excretory-secretory (TES) antigen. J Parasit Dis 40, 1038–1043 (2016). https://doi.org/10.1007/s12639-014-0630-4

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  • DOI: https://doi.org/10.1007/s12639-014-0630-4

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