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Sex dependent alterations in the protein characterization patterns of Haemonchus contortus

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Abstract

The aim of the study was to highlight the sex dependent differences in the electrophoretic protein patterns of male and female Haemonchus contortus worms SDS based polyacrylamide gels of both male and female worms were run side by side for comparison. A total of 33 and 35 polypeptides were detected in polyacrylamide gels stained with coomassie brilliant blue R-250, respectively. Besides many of the fundamental homologies in protein profile, some of the polypeptides specific to either sex were also observed. Most of the characteristic polypeptides were of low molecular weight. These polypeptides needs deeper unrevealing regarding the nature of protein, through well planned zymographic studies, so as to ascertain the true nature and/or type of protein involved in those bands. This will help us in better understanding of parasite immunology and sex influenced differences amongst the worm and the possible variations in their pathogenesis contributed thereof, if any.

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References

  • Coles GC (2002) Cattle nematodes resistant to anthelmintics: why so few cases? Vet Res 33:481–489

    Article  CAS  PubMed  Google Scholar 

  • Dzandu JK, Mercy ED, Eenise LB, Garry EW (1984) Detection of erythrocyte membrane protein, sialoglycoprotein, and lipids in the same polyacrylamide gel using a double staining technique. Proc Natl Acad Sci 81:1733–1737

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Irie S, Seizaki M, Kato Y (1982) A faithful double stain of proteins in the polyacrylamide gels with coomassie blue and silver. Anal Biochem 126:350–354

    Article  CAS  PubMed  Google Scholar 

  • Jaiswal AK, Sudan V, Shanker D, Kumar P (2013) Emergence of ivermectin resistance in gastrointestinal nematodes of goats in a semi-organized farm of Mathura district—India. Vet Arhiv 83(3):275–280

    CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  • Saifullah MK, Ali A, Abidi SMA, Nizami WA (1993) Sex dependent differences in transaminases and polypeptides of Ascaridia galli. J Parasit Appl Anim Biol 2:19–25

    Google Scholar 

  • Shoiab M, Irshadullah M (2009) Protein profile of male and female Haemonchus contortus. J Vet Parasitol 23(2):147–149

    Google Scholar 

  • Sibley CG (1960) The electrophoretic patterns of avian egg white proteins as taxonomic characters. IBIS 102:215–284

    Article  Google Scholar 

  • Smith WD (1999) Prospects for vaccines of helminth parasites of grazing ruminants. Int J Parasitol 29:17–24

    Article  CAS  PubMed  Google Scholar 

  • Sood ML, Kapur J (1982) Electrophoretic analysis of proteins of knobbed and linguiform morphs of female Heamonchus contortus Nematoda: (Trichostrongylidae). Helminthologia 19:273–278

    Google Scholar 

  • Sudan V, Tewari AK, Singh H, Sarvanan BC (2014) Prokaryotic expression and molecular characterization of surface antigen 3 (SAG 3) protein of Toxoplasma gondii. Indian J Anim Sci 84(8):824–828

    CAS  Google Scholar 

Download references

Acknowledgments

The authors are highly thankful to the Vice Chancellor, DUVASU for the facilities provided.

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Authors and Affiliations

  1. Department of Veterinary Parasitology, College of Veterinary Sciences & Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India

    Amit Kumar Jaiswal, Vikrant Sudan, Amit Singh, Ruchi Singh Gaur, Dharmendra Kanojiya & Daya Shanker

  2. Department of Biochemistry, College of Veterinary Sciences & Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India

    Vijay Pandey & Rajesh Nigam

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  1. Amit Kumar Jaiswal
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  2. Vikrant Sudan
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  3. Vijay Pandey
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  4. Amit Singh
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  5. Ruchi Singh Gaur
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  6. Dharmendra Kanojiya
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  7. Rajesh Nigam
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  8. Daya Shanker
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Correspondence to Vikrant Sudan.

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Jaiswal, A.K., Sudan, V., Pandey, V. et al. Sex dependent alterations in the protein characterization patterns of Haemonchus contortus . J Parasit Dis 40, 1006–1008 (2016). https://doi.org/10.1007/s12639-014-0624-2

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  • DOI: https://doi.org/10.1007/s12639-014-0624-2

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