Abstract
Lipid classes and fatty acid compositions of a digenetic trematode, Isoparorchis hypselobagri inhabiting swim bladder of Wallago attu were analyzed by TLC and GLC. The total lipid (TL) and percent content of neutral lipid (NL, 61.59), glycolipid (GL, 19.78) and phospholipid (PL, 18.62) were recorded. The total lipid is 3.81 % of the wet weight of the body tissue. The total percent of saturates, monoenes, dienes and polyenes in TL, NL, GL, and PL of the parasite were 52.0, 49.7, 57.9, 61.5; 31.7, 31.8, 33.1, 26.3; 5.7, 5.7, 4.7, 4.2; as well as 10.33, 12.6, 4.3, 7.4 respectively. Diunsaturated fatty acids were present in low amount. Palmitic 16:0 (among all saturates) and oleic 18:1ω9 (among all unsaturates) acids were the predominant fatty acids in the parasite recorded.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ackman RG (2000) Fatty acids in fish and shellfish. In: Chow CK (ed) Fatty acids in foods and their health implications. M. Dekker, New York, pp 153–172
Ackman RG, Burgher RD (1965) Cod liver oil fatty acids as secondary reference standards in the GLC of polyunsaturated fatty acid of animal origin: analysis of a dermal oil of the Atlantic leather-back turtle. J Am Oil Chem Soc 42:38–42
Awharitoma AO, Opute FI, Ali SN, Obiamiwe BA (1988) Lipid composition of four species of amphistomes (Trematoda) from the rumen of cattle. Int J Parasitol 18:441–444
Barrett J (1981) Biochemistry of parasitic helminths. University Park Press, Baltimore
Bennett MW, Caulfield JP (1991) Specific binding of human low density lipoprotein to the surface of schistosomula of Schistosoma mansoni and ingestion by the parasite. Am J Pathol 138:1173–1182
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917
Cheng TC (1986) General parasitology, 2nd edn. Academic Press, Orlando
Christie WW (2003) Lipid analysis, 3rd edn. Oily Press, Bridgwater
Ezigbo JC, Onyeneke EC (1987) Studies on the energy reserve of Fasciola gigantica (COBB.) from Nigerian zebu cattle. II. The fatty acid composition. Beitr Trop Landwirtsch Veterinarmed 25(3):343–346
Furlong ST (1991) Unique roles for lipids in Schistosoma mansoni. Parasitol Today 7:59–62
Ghosh D, Dey C, Misra KK (2005) Host–parasite relationship: fatty acid compositions of trematode, Paramphistomum cervi and common Indian goat Capra hircus. J Parasit Dis 29(2):119–123
Goil MM (1964) Physiological studies on trematodes. Lipid fraction in Gastrothylax crumenifer. Parasitology 54:81–85
Meyer F, Kimura S, Mueller JF (1966) Lipid metabolism in the larval and adult forms of the tapeworm Spirometra mansonoides. J Biol Chem 241:4224–4232
Oldenborg V, Van Vugt F, Van Goldge LMG (1975) Composition and metabolism of phospholipids of Fasciola hepatica, the common liver fluke. Biochim Biophys Acta 398:101–110
Roberts LS, Janovy J Jr (eds) (2000) Foundation of parasitology, 6th edn. Mc-Graw Hill, International Edition, Boston
Rouser G, Kritchevsky G, Yamamoto A (eds) (1967) Lipid chromatographic analysis, 2nd edn. Marcel Dekker Inc, New York
Smith MT, Jr Brooks TJ (1969) Lipid fractions in adult Schistosoma mansoni. Parasitology 59:293–298
Smyth JD (1994) Animal parasitology. Cambridge University Press, Cambridge
Smyth JD & Halton DW (1983) The physiology of trematodes. 2nd Edition. Cambridge University Press, ISBN 0 521 29434 7
Tielens AGM (1997) Biochemistry of trematodes. In: Fried B, Graczyk TK (eds) Advances in trematode biology. CRC Press, Boca Raton, pp 309–343
Tunholi-Alves VM, Tunholi VM, Gôlo P, Lustrino D, Maldonado A Jr, Bittencourt VR, Rodrigues Mde L, Pinheiro J (2011) Lipid levels in Biomphalaria glabrata infected with different doses of Echinostoma paraensei miracidia. Exp Parasitol 128(3):212–216
Von Brand Th (1973) Biochemistry of Parasites. Academic Press, New York
Acknowledgments
The authors are thankful to the Principal, Serampore College, Hooghly for providing necessary laboratory facilities. Authors extending their heartfelt thanks to Dr. Amitava Ghosh, the Drug Research and Development Centre, Dum Dum, Kolkata for GLC analysis of the sample.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mondal, J., Dey, C. Lipid and fatty acid compositions of a trematode, Isoparorchis hypselobagri Billet, 1898 (Digenea: Isoparorchiidae) infecting swim bladder of Wallago attu in the district North 24-Parganas of West Bengal. J Parasit Dis 39, 67–72 (2015). https://doi.org/10.1007/s12639-013-0283-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12639-013-0283-8