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Recovery of Verticillium dahliae from commercially available potato seed lots planted in Turkey and characterization of isolates by vegetative compatibility and aggressiveness

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Abstract

A total of 105 seed samples collected from commercially available potato seed lots in Turkey were assayed for Verticillium dahliae. V. dahliae was successfully isolated from 29 of the 105 seed lots tested. The frequency of infected lots was nearly 28%. Vegetative compatibility of the isolates was assessed through complementation tests using nitrate non-utilizing mutants. Of the 110 isolates obtained, 63 were assigned to VCG4A, 24 to VCG4B, 19 to VCG2A and one to VCG2B, while the three remaining isolates could not be assigned to any of the identified VCGs. All 36 of the isolates tested in the greenhouse on potato cv. ‘Russet Burbank’ were pathogenic to potato. As a group, AUDPC values were significantly higher (P <0.05) for VCG4A than for VCG4B and VCG2 isolates. These data suggest that (i) commercial potato seed lots are commonly infected with V. dahliae, and that this is a primary method by which the pathogen can be introduced into production fields; (ii) potato isolates of V. dahliae belong to VCG4A, 4B and 2A and these isolates are widely distributed via seed lots; and (iii) VCG4A and VCG4B are distinct pathotypes of V. dahliae that vary in their aggressiveness to potato. The present study is the first report of natural infections of potato by VCG4A and VCG2A in Turkey.

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Acknowledgments

Our thanks are expressed to Mr. Adnan Özkan for technical assistance. This research was supported by funding from the Abant Izzet Baysal University Research Program (BAP/2012.24.01.562).

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Authors and Affiliations

  1. Abant Izzet Baysal University, Faculty of Agriculture and Natural Sciences, Department of Plant Protection, Bolu, Turkey

    Mehmet Erhan Göre

  2. Bozok University, Faculty of Agriculture and Natural Sciences, Department of Field Crops, Yozgat, Turkey

    Belgin Coşge Şenkal

  3. Abant Izzet Baysal University, Vocational Higher School, Mudurnu, Bolu, Turkey

    Selma Kuru Berk & Selma Tuna

  4. Potato Research Station, Niğde, Turkey

    Hüseyin Onaran

  5. Agricon Pesticide Experiment and Consultancy, İzmir, Turkey

    Nedim Altin

  6. Abant Izzet Baysal University, Faculty of Arts and Science, Department of Biology, Bolu, Turkey

    Elif Ay & Nusret Zencirci

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  1. Mehmet Erhan Göre
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Correspondence to Mehmet Erhan Göre.

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Göre, M.E., Şenkal, B.C., Berk, S.K. et al. Recovery of Verticillium dahliae from commercially available potato seed lots planted in Turkey and characterization of isolates by vegetative compatibility and aggressiveness. Phytoparasitica 43, 241–251 (2015). https://doi.org/10.1007/s12600-014-0436-z

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  • DOI: https://doi.org/10.1007/s12600-014-0436-z

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