Abstract
Poecillastra laminaris (Sollas) (Order Tetractinellida, Family Vulcanellidae) is a demosponge widely distributed on seamounts and other habitats around the New Zealand region. In order to investigate its genetic structure and connectivity, ten polymorphic microsatellite markers were developed and tested on all 54 individuals in the NIWA Invertebrate Collection (NIC), Wellington, using low-cost M13 labelling. High quality results were obtained for 34 individuals. The microsatellite loci were highly polymorphic, with ten to 27 alleles per locus (mean ± SD of 16.2 ± 5.1). Expected and observed heterozygosities of these loci were 0.786 to 0.952 and 0.550 to 0.926, respectively. These microsatellite markers will be used for population genetic studies of P. laminaris, in particular relating to the identification of metapopulations and barriers to gene flow. Results will inform spatial management planning for the protection of vulnerable marine ecosystems.
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Acknowledgments
Sponge material was collected by NIWA and supplied for genetic work by the NIWA Invertebrate Collection. We are grateful to Sadie Mills and Kareen Schnabel, NIWA Invertebrate Collection, Wellington, for their diligent assistance with loans, and Ashley Rowden and Malcolm Clark of NIWA for comments on an earlier draft of this manuscript. The study was supported by funding from the New Zealand Ministry of Business, Innovation and Employment as part of the NIWA-led project “Predicting the occurrence of vulnerable marine ecosystems for planning spatial management in the South Pacific region” (CO1X1229). This research was also funded by NIWA under the Coasts and Oceans Research Programme 2 Marine Biological Resources: Discovery and definition of the marine biota of New Zealand (2014/2015 & 2015/2016 SCIs).
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Communicated by P. Martinez Arbizu
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Zeng, C., Kelly, M. & Gardner, J.P.A. Development and characterization of ten highly polymorphic microsatellite markers for the demosponge Poecillastra laminaris (Sollas). Mar Biodiv 48, 1265–1267 (2018). https://doi.org/10.1007/s12526-016-0540-z
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DOI: https://doi.org/10.1007/s12526-016-0540-z