Skip to main content

Advertisement

SpringerLink
Log in

Microsatellite loci to determine population structure in the yellow seahorse (Hippocampus kuda) and the three-spotted seahorse (H. trimaculatus)

  • Short Communication
  • Published:
Marine Biodiversity Aims and scope Submit manuscript

Abstract

Genetic diversity of seahorse species was studied using 12 polymorphic microsatellite DNA loci in the yellow seahorse Hippocampus kuda and the three-spotted seahorse H. trimaculatus from samples collected along the east and west coasts of India. Cross-species microsatellite loci amplification in populations of Indian seahorses showed significant numbers of private alleles associated with site-specific populations, which could be used for population genetic studies and management of species for conservation. Significant genotype heterogeneity indicated that the samples are not from the same gene pool, and that physical barriers exist which inhibit breeding between populations. The identified microsatellite loci can be further utilized in fine-scale population structure analysis of seahorses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Belkhir K, Borsa P, Chikhi L, Goudet J, Bonhomme F (1997)GENETIX 3.07, WindowsTMSoftware for Population Genetics.Laboratoire Génome et Populations, University of Montpellier II,Montpellier

  • Boulenger GA (1900) Descriptions of a new seahorse (Hippocampus) from Muscat. Ann Mag Nat Hist 7(6):51–52

    Google Scholar 

  • Bovo G, Nishizawa T, Maltese C, Borghesan F, Mutinelli F, Montesi F, De Mas S (1999) Viral encephalo-retinopathy of farmed fish species in italy. Virus Res 63:143–146

    Article  PubMed  CAS  Google Scholar 

  • Cornuet JM, Luikart G (1996) Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics 144:2001–2014

    PubMed  CAS  Google Scholar 

  • Ellegren H (2004) Microsatellites: simple sequences with complex evolution. Nat Rev Genet 5:435–445

    Article  PubMed  CAS  Google Scholar 

  • Foster SJ, Vincent ACJ (2004) Life history and ecology of seahorse: implications for conservation and management. J Fish Biol 65:1–61

    Article  Google Scholar 

  • Galbusera PHA, Gillemot S, Jouk P, Teske PR, Hellemans B, Volckaert FAMJ (2007) Isolation of microsatellite markers for the endangered Knysna seahorse Hippocampus capensis and their use in the detection of a genetic bottleneck. Mol Ecol Notes 7(4):638–640

    Article  CAS  Google Scholar 

  • Gopalakrishnan A, Musammilu KK, Muneer PMA, Lal KK, Kapoor D, Ponniah AG, Mohindra V (2004) Microsatellite DNA markers to assess population structure of red-tailed barb, Gonoproktopterus curmuca. Acta Zool Sin 50(4):686–690

    CAS  Google Scholar 

  • Jones AG, Kvarnemo C, Moore GI, Simmons LW, Avise JC (1998) Microsatellite evidence for monogamy and sex-biased recombination in the Western Australian seahorse Hippocampus angustus. Mol Ecol 7:1497–1505

    Google Scholar 

  • Kuiter RH (2000) Seahorses, pipefishes and their relatives: a comprehensive guide to Syngnathiformes. TMC, Chorleywood

    Google Scholar 

  • Lal KK, Chauhan T, Mandal A, Singh RK, Khulbe L, Ponniah AG, Mohindra V (2004) Identification of microsatellite DNA markers for population structure analysis in Indian major carp, Cirrhinus mrigala (Hamilton-Buchanan, 1882). J Appl Ichthyol 20(2):87–91

    Article  CAS  Google Scholar 

  • Langen K, Schwarzer J, Kullmann H, Bakker TCM, Thünken T (2011) Microsatellite support for active inbreeding in a cichlid fish. PLoS One 6(9):e24689. doi:10.1371/journal.pone.0024689

    Article  PubMed  CAS  Google Scholar 

  • Lessios HA (1992) Testing electrophoretic data for agreementwith Hardy-Weinberg expectations. Mar Biol 112:517–523

    Google Scholar 

  • Louie KD, Bardeleben C (2006) Isolation of polymorphic tetranucleotide microsatellite markers for the bay pipefish Syngnathus leptorhynchus.Mol Ecol Notes 6:1117–1118

    Google Scholar 

  • Lourie SA, Randall JE (2003) A New Pygmy Seahorse, Hippocampus denise (Teleostei: Syngnathidae), from the Indo-Pacific. Zool Stud 42(2):284–291

    Google Scholar 

  • Lourie SA, Pritchard JC, Casey SP et al (1999a) The taxonomy of Vietnam’s exploited seahorses. Biol J Linn Soc 66:231–256

    Article  Google Scholar 

  • Lourie SA, Vincent ACJ, Hall HH (1999b) Seahorse: an identification guide to the world’s species and their conservation. Project Seahorse, London

    Google Scholar 

  • Lourie SA, Green DM, Vincent ACJ (2005) Dispersal, habitat differences, and comparative phylogeography of South East Asian seahorses (Syngnathidae: Hippocampus). Mol Ecol 14:1073–1094

    Article  PubMed  CAS  Google Scholar 

  • Mohindra V, Khulbe L, Lal KK, Ponniah AG (2001b) Sequence of microsatellite locus L roh G1 in Labeo rohita. Accession and Locus # AF415207. NCBI sequence information, National Centre for Biotechnology Information. www.ncbi.nlm.nih.gov

  • Mohindra V, Mishra A, Palanichamy M, Ponniah AG (2001b) Cross-species amplification of Catla catla microsatellite locus in Labeo rohita. Indian J Fish 48(1):103–108

    Google Scholar 

  • Moore SS, Sargeant LL, King TJ, Mattick JS, Georges M, Hetzel DJS (1991) The conservation of dinucleotide microsatellites among mammalian genomes allows use of heterologous PCR primer pairs in closely related species. Genomics 10:654–660

    Article  PubMed  CAS  Google Scholar 

  • Neff BD, Gross MR (2001) Microsatellite evolution in vertebrates: inference from AC dinucleotide repeates. Evolution 55(9):1717–1733

    PubMed  CAS  Google Scholar 

  • O’Connell, Wright (1997) Microsatellite DNA in fishes. Rev Fish Biol Fish 7:331–363

    Article  Google Scholar 

  • Pardo BG, Lopez A, Martinez P, Bouza C (2007) Novel microsatellite loci in the threatened European long- snouted seahorse (Hippocampus guttulatus) for genetic diversity and parentage analysis. Conserv Genet 8(5):1243–1245

    Article  CAS  Google Scholar 

  • Rousset F, Raymond M (1995) Testing heterozygote excess and deficiency. Genetics 140:1413–1419

    PubMed  CAS  Google Scholar 

  • Scribner KT, Gust JR, Fields RL (1996) Isolation and characterization of novel amplification salmon microsatellite loci: cross-species and population genetic applications. Can J Fish Aquat Sci 53:833–841

    Article  CAS  Google Scholar 

  • Slatkin M (1995) A measure of population subdivision based on microsatellite allele frequencies. Genetics 139:457–462

    PubMed  CAS  Google Scholar 

  • van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro–checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • Van Oosterhout C, Weetman D, Hutchinson WF (2006) Estimation and adjustment of microsatellite null alleles in non equilibrium populations. Mol Ecol Notes 6:255–256

    Article  Google Scholar 

  • Vincent ACJ (1996) The international trade in seahorses. TRAFFIC International, Cambridge

    Google Scholar 

  • Wang D, Shi J, Carlson SR, Cregan PB, Ward RW, Diers BW (2003) A low-cost, high-throughput polyacrylamide gel electrophoresis system for genotyping with microsatellite DNA marker. Crop Sci 43:1828–1832

    Article  CAS  Google Scholar 

  • Weir B, Cockerham C (1984) Estimating F statistics for the analysis of populationstructure. Evolution 38:1358–1370

    Google Scholar 

  • Zhang C-G, Cai B, Xu T-Q (1995) Fishes and fish resources in Xizang, China. China Agriculture Press, Beijing

  • Zardoya R, Vollmer DM, Craddock C, Streelman JT, Karl S, Meyer A (1996) Evolutionary conservation of microsatellite flanking regions and their use in resolving the phylogeny of cichlid fishes (Pisces: Perciformes). Proc R Soc Lond B 263:1589–1598

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank DG, ICAR and staff at NBFGR Cochin Unit for providing facilities, and the staff at various research labs who provided whole-hearted support.

Author information

Authors and Affiliations

  1. NBFGR, Lucknow, Uttar Pradesh, India

    Karan veer Singh & Wazir Singh Lakra

  2. NBFGR, Cochin Unit-NBFGR, Cochin, India

    A. Gopalakrishnan

  3. Punjab University Chandigarh, Chandigarh, India

    R. C. Sobti

Authors
  1. Karan veer Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Gopalakrishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wazir Singh Lakra
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. C. Sobti
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Karan veer Singh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

veer Singh, K., Gopalakrishnan, A., Lakra, W.S. et al. Microsatellite loci to determine population structure in the yellow seahorse (Hippocampus kuda) and the three-spotted seahorse (H. trimaculatus). Mar Biodiv 42, 481–488 (2012). https://doi.org/10.1007/s12526-012-0122-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12526-012-0122-7

Keywords

Search

Navigation