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Stenotrophomonas maltophilia HW2 enhanced cucumber resistance against cucumber green mottle mosaic virus

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Abstract

Cucumber green mottle mosaic virus (CGMMV) is a major limiting factor in the production of cucumber plants worldwide. In the present study, we use plant growth-promoting rhizobacteria (PGPR) to control this virus effectively. Stenotrophomonas maltophilia HW2 was isolated from healthy cucumber root, exhibited a good biocontrol efficacy against CGMMV. Here, it is documented that 20 d after virus inoculation, the biocontrol efficacy of HW2 reached 52.61%. HW2 can effectively colonize in cucumber rhizosphere, and also promoted cucumber plants growth. We also examined the effect of HW2 on viral replication and its mechanism. Compared with the control, HW2 pre-treated plants could delay virus replication for more than 3 d and inhibit viral protein genes (CP, MP, Rep) expression in the cucumber leaf. The expression of antioxidant enzyme genes (SOD and CAT) and defense-related genes (PR1 and PR5) were quickly induced by HW2. These results suggest that HW2 induced plant defense responses to CGMMV by increasing the expression of defense response genes. We report for the first time that Stenotrophomonas maltophilia improved cucumber resistance against CGMMV, which highlights the applying of PGPR on controlling of virus diseases.

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Authors and Affiliations

  1. Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China

    Hongwei Li, Wenxiang Huang, Long Xu, Xiaosi Zhou & Hongxia Liu

  2. Key Laboratory of Integrated Pest Management in Crops in Eastern China (Nanjing Agricultural University), Ministry of Agriculture, Nanjing, 210095, China

    Hongwei Li, Wenxiang Huang, Long Xu, Xiaosi Zhou & Hongxia Liu

  3. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Zhaobang Cheng

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  1. Hongwei Li
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  2. Wenxiang Huang
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  3. Long Xu
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  4. Xiaosi Zhou
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  5. Hongxia Liu
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  6. Zhaobang Cheng
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Correspondence to Hongxia Liu.

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Li, H., Huang, W., Xu, L. et al. Stenotrophomonas maltophilia HW2 enhanced cucumber resistance against cucumber green mottle mosaic virus. J. Plant Biol. 59, 488–495 (2016). https://doi.org/10.1007/s12374-016-0246-6

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  • DOI: https://doi.org/10.1007/s12374-016-0246-6

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