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Identification of Differentially Expressed Genes in Flower Buds of Calanthe discolor and C. sieboldii

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Abstract

The genus Calanthe includes species of terrestrial orchids that produce attractive flowers with diverse floral traits. Breeding programs have been established to improve the horticultural value of various Calanthe species, but studies to identify the genetic components contributing to the key phenotypic characteristics have not been undertaken. To understand the molecular mechanisms underlying floral development associated with floral morphology, color, and fragrance production, the flower buds of two typical Korean Calanthe species, C. discolor and C. sieboldii, were subjected to gene expression analysis by differential display RT-PCR (DDRT-PCR). A total of 66 non-redundant differentially expressed genes (DEGs) were isolated and sequenced. Of these, 26 and 40 DEGs were found to be highly expressed in C. discolor and C. sieboldii, respectively. Moreover, the expression patterns of a subset of genes presumably implicated in signal transduction, metabolic pathways, and hormonal signaling differed between the two species. The data presented here may improve our understanding of the mechanisms underlying floral development and contribute to advances in orchid biotechnology.

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Abbreviations

DEG:

Differentially expressed gene

RT-PCR:

Reverse transcription-polymerase chain reaction

ACP:

Annealing control primer

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Acknowledgements

We thank Dr. Hwang Inhwan (POSTECH, Korea) for providing the pBluescript-T vector. This work was supported by research funds from Chonbuk National University in 2007 (NP-2007-180103), the Korea Research Foundation Grant (KRF2007-412-J05501), and a grant from the Jeonbuk Forest Environment Research Institute.

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Authors and Affiliations

  1. Jeonbuk Forest Environment Research Institute, Jeonju, 560-120, South Korea

    Joon Moh Park

  2. Division of Science Education, Chonbuk National University, Jeonju, 561-756, South Korea

    Sung Soo Whang & Ja Choon Koo

  3. Division of Biological Sciences, Chonbuk National University, Jeonju, 561-756, South Korea

    Soonku So

  4. Department of Science Education, Jeju National University, Jeju, 690-756, South Korea

    Pyung Ok Lim

  5. Department of Biotechnology, Jeju National University, Jeju, 690-756, South Korea

    Hyo-Yeon Lee

  6. Subtropical Horticulture Research Institute, Jeju National University, Jeju, 690-756, South Korea

    Pyung Ok Lim, Hyo-Yeon Lee & Ja Choon Koo

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  1. Joon Moh Park
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  2. Sung Soo Whang
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  3. Soonku So
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Correspondence to Ja Choon Koo.

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Park, J.M., Whang, S.S., So, S. et al. Identification of Differentially Expressed Genes in Flower Buds of Calanthe discolor and C. sieboldii . J. Plant Biol. 53, 24–31 (2010). https://doi.org/10.1007/s12374-009-9082-2

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  • DOI: https://doi.org/10.1007/s12374-009-9082-2

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