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Artificial Inoculation Method of Pokkah Boeng Disease of Sugarcane and Screening of Resistant Germplasm Resources in Subtropical China

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Abstract

Evaluation of four different methods of artificial inoculation viz., syringe and spindle inoculation, detached leaf assay and sett dip methods indicated that the syringe method was the most suitable followed by spindle inoculation for evaluation of sugarcane varieties against pokkah boeng disease under controlled conditions. However, the preliminary assessment of the resistance of 88 sugarcane germplasm lines against two most prevalent fungal species associated with pokkah boeng disease, F. verticillioides CNO-1 and F. proliferatum YN-41, by spindle inoculation method indicated that the CNO-1 was more virulent. Furthermore, our study identified GT11, GT37, NCO310, POJ2878, and F134 as highly susceptible, whereas CP84-1198, GT05-3846, ROC27, and YT94-128 as highly resistant clones. Spindle inoculation method was found as the most reliable method of inoculation of young sugarcane germplasm under indoor condition for screening resistance against pokkah boeng disease.

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References

  • Aoki, T., K. O’Donnell, and D.M. Geiser. 2014. Systematics of key phytopathogenic Fusarium species: Current status and future challenges. Journal of General Plant Pathalogy 80(3): 189–201.

    Article  CAS  Google Scholar 

  • Appunu, C., and M.N. Premachandran. 2012. Choice of parental clones in sugarcane breeding programme at Coimbatore in evolving Co canes—A retrospection. In Proceedings of the international symposium on new paradigms in sugarcane research, ed. R. Viswanathan, G. Hemaprabha, A. Bhaskaran, K. Mohanraj, V. Jayakumar, T. Ramasubramanian, and N.V. Nair, 11–12. Coimbatore: Sugarcane Breeding Institute.

    Google Scholar 

  • Balamuralikrishna, M., D. Sabitha, T. Ganapathy, and R. Viswanathan. 2003. Sugarcane mosaic virus infection progress in relation to age of sugarcane. Sugar Tech 5(1&2): 21–24.

    Article  Google Scholar 

  • Chen, R.K., L.P. Xu, and Y.Q. Lin. 2011. Modern sugarcane genetic breeding. Beijing: China Agriculture Press.

    Google Scholar 

  • Gazaffi, R., K.M. Oliveira, A.P. Souza, and A.A.F. Garia. 2010. The importance of the germplasm in developing agro-energetic profile sugarcane cultivars. In Sugar Cane Bioethanol: R&D for productivity and sustainability, ed. L.A.B. Cortez, 333–343. Sao Paulo: Blucher.

    Google Scholar 

  • Govender, P., S.A. McFarlane, and R. Rutherford. 2010. Fusarium species causing pokkah boeng and their effect on Eldana saccharina Walker (lepidoptera: pyralidae). Proceedings of the South African Sugar Technology Association 83:267–270.

    Google Scholar 

  • Hapase, R.S. 2012. Breeding strategies for improving productivity and diseases resistance. In Sugarcane breeders and pathologist meet, ed. N.V. Nair, R. Viswanathan, P. Govindaraj, and K. Mohanraj, 86–87. Sugarcane Breeding Institute: Coimbatore.

    Google Scholar 

  • Khani, K.T., A. Alizadeh, R.F. Nejad, and A.S Tehrani. 2013. Pathogenicity of Fusarium proliferatum, a new causal agent of pokkah boeng in sugarcane. Proceedings of the International Society Sugarcane Technology 23: 1–5.

    Google Scholar 

  • Li, Y.R. 2003. Discussion about the sugarcane sugar industry development in Guangxi after China’s entry into WTO. Guangxi Agricultural Sciences 1: 1–4.

    Google Scholar 

  • Li, Y.R. 2005. Springing up of sugarcane and sugar industry in China. Guangxi Agricultural Sciences 36(1): 79–81.

    Google Scholar 

  • Lyrene, P.M., J.L. Dean, and N.I. James. 1977. Inheritance of resistance to pokkah boeng in sugarcane crosses. Phytopathology 67: 689–692.

    Article  Google Scholar 

  • Lin, Z.Y., S.Q. Xu, Y.X. Que, J.H. Wang, and J.C. Comstock. 2014. Species-specific detection and identification of Fusarium species complex, the causal agent of sugarcane pokkah boeng in China. PLoS ONE 9(8): e104195. doi:10.1371/journal.pone.0104195.

    Article  PubMed  PubMed Central  Google Scholar 

  • Martin, J.P., H. Handojo, and C. Wismer. 1989. Pokkah Boeng-diseases of sugarcane. Diseases of Sugarcane xi: 157–168.

    Article  Google Scholar 

  • McFarlane, S.A., and R. Rutherford. 2005. Fusarium species isolated from sugarcane in KwaZulu-Natal and their effect on Eldana saccharina (Lepidoptera: Pyralidae) development in vitro. In Proceedings of the 79th annual congress of South African Sugar Technologists’ Association, held at Kwa-Shukela, Mount Edgecombe, South Africa, 19–22 July 2005.

  • Olweny, C., N. Kahiu, H. Nzioki, and S.M. Githiri. 2008. Evaluation of smut inoculation techniques in sugarcane seedlings. Sugar Tech 10(4): 341–345.

    Article  Google Scholar 

  • Patil, A.S. 1995. Studies on pokkah boeng and pine apple disease of sugarcane in Maharashtra with their economic losses in yield and quality of sugarcane. Final Project Report. Pune: ICAR, VSI.

    Google Scholar 

  • Prom, L.K., B.J. Steffenson, B. Salas, J. Mos, Fetch, T.G. Jr, and H.H. Casper. 1996. Evaluation of selected barley accessions for resistance to Fusarium head blight and deoxynivalenol concentration. In G. Scoles and B. Rossnagel (ed.) Proceedings of the Fifth Internatinal Oat Conference and VII International Barley Symposium, 764–766. Saskatoon, Canada. 30 July–6 August 1996.

  • Rao, J.T. 1989. Sugarcane Origin, Taxonomy, Breeding and Varieties. In Sugarcane varietal improvement, ed. K.M. Naidu, T.V. Sreenivasan, and M.N. Premachandran, 83–113. Coimbatore: Sugarcane Breeding Institute.

    Google Scholar 

  • Singh, R.K., M.S. Khan, R. Singh, D.K. Pandey, S. Kumar, and S. Lal. 2011. Analysis of genetic differentiation and phylogenetic relationships among sugarcane genotypes differing in response to red rot. Sugar Tech 13(2): 137–144.

    Article  Google Scholar 

  • Singh, A., S.S. Chauhan, A. Singh, and S.B. Singh. 2006. Deterioration in sugarcane due to pokkah boeng disease. Sugar Tech 8: 187–190.

    Article  CAS  Google Scholar 

  • Siddique, S. 2007. Pathogenicity and aethiology of Fusarium species associated with pokkah boeng disease on sugarcane. Thesis, University of Malaysia, Malaysia.

  • Vishwakarma, S., P. Kumar, A. Nigam, A. Singh, and A. Kumar. 2013. Pokkah boeng: an emerging disease of sugarcane. Journal of Plant Pathology and Microbiology 4: 170. doi:10.4172/2157-7471.1000170.

    Google Scholar 

  • Whittle, P., and L. Irawan. 2000. Pokkah boeng. In A guide to sugarcane diseases, ed. P. Rott, R.A. Bailey, J.C. Comstock, B.J. Croft, and A.S. Saumtally, 136–140. Montpellier: CIRAD and ISSCT.

    Google Scholar 

  • Wang, Z.P., W.X. Duan, Y.J. Li, Q. Liang, Z.G. Zhou, M.Q. Zhang, and S.H. Lin. 2016. Establishment of resistance evaluation system in the field for sugarcane pokkah boeng. Journal of South China Agricultural University 37(3): 67–72.

    Google Scholar 

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Authors and Affiliations

  1. Agricultural College, State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530004, China

    Ze-Ping Wang, Hai-Jun Sun, Qiang Guo, Shi-Qiang Xu, Ji-Hua Wang & Mu-Qing Zhang

  2. Guangxi Key Laboratory of Sugarcane Genetic Improvement and Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Institute of Guangxi Academy of Agricultural Sciences-Sugarcane Research Center of Chinese Academy of Agricultural Sciences, Nanning, 530007, China

    Ze-Ping Wang, Shan-Hai Lin & Mu-Qing Zhang

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  1. Ze-Ping Wang
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  2. Hai-Jun Sun
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  3. Qiang Guo
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  4. Shi-Qiang Xu
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  5. Ji-Hua Wang
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  6. Shan-Hai Lin
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  7. Mu-Qing Zhang
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Correspondence to Mu-Qing Zhang.

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Wang, ZP., Sun, HJ., Guo, Q. et al. Artificial Inoculation Method of Pokkah Boeng Disease of Sugarcane and Screening of Resistant Germplasm Resources in Subtropical China. Sugar Tech 19, 283–292 (2017). https://doi.org/10.1007/s12355-016-0465-7

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  • DOI: https://doi.org/10.1007/s12355-016-0465-7

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