Skip to main content
SpringerLink
Log in

Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane

  • Short Communication
  • Published:
Sugar Tech Aims and scope Submit manuscript

Abstract

Commercial sugarcane cultivars and wild species have a high ploidy level allied to a complex genome and usual chromosomal instability. There are few studies related to the standardization of cytogenetic techniques in sugarcane. This work tested the efficiency of three antimitotics (colchicine, 8-hydroxyquinoline, and trifuralin) at different concentrations on the quality of metaphases spreads from a commercial sugarcane cultivar (IAC911099-Saccharum spp.) and a wild sugarcane accession (Krakatau-S. spontaneum). Although all three antimitotics were efficient for obtaining metaphases, 8-hydroxyquinoline was the most efficient for both IAC911099 and Krakatau. The chromosome number of these two genotypes was inferred. The variation in chromosome number was 2n = 90–112 for IAC911099, with a statistical modal of 2n = 112 chromosomes while the variation in chromosome number was 2n = 90–129 chromosome with a statistical modal of 2n = 128 chromosomes for Krakatau (S. spontaneum).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

References

  • Barreto, Adelaide, and Jean-Pierre Simon. 1982. Identificação de progenies y progenitores por el análisis del numero cromossomico em Saccharum. Turrialba 32(3): 321–327.

    CAS  Google Scholar 

  • Bonasora, Marisa Graciela, Lidia Poggio, and Eduardo José Greizerstein. 2013. Cytogenetic studies in four cultivated Amaranthus (Amaranthaceae) species. Comparative Cytogenetics 7(1): 53.

    Article  PubMed  PubMed Central  Google Scholar 

  • Cardoso, Danon Clemes, Maykon Passos Cristiano, Luísa Antônia Campos Barros, Denilce Meneses Lopes, and Silvia das Graças Pompolo. 2012. First cytogenetic characterization of a species of the arboreal ant genus Azteca Forel, 1978 (Dolichoderinae, Formicidae). Comparative Cytogenetics 6(2): 107.

    Article  PubMed  PubMed Central  Google Scholar 

  • Cuadrado, A., R. Acevedo, S. Moreno Diaz de la Espina, N. Jouve, and C. De La Torre. 2004. Genome remodelling in three modern S. officinarum × S. spontaneum sugarcane cultivars. Journal of Experimental Botany 55(398): 847–854.

    Article  CAS  PubMed  Google Scholar 

  • Cuco, Silvia Marina, Mateus Mondin, Maria Lúcia Carneiro Vieira, and Margarida L.R. Aguiar-Perecin. 2003. Técnicas para a obtenção de preparações citológicas com alta freqüência de metáfases mitóticas em plantas: Passiflora (Passifloraceae) e Crotalaria (Leguminosae). Acta Botanica Brasilica 17(3): 363–370.

    Article  Google Scholar 

  • D’Hont, Angelique, L. Grivet, P. Feldmann, J.C. Glaszmann, S. Rao, and N. Berding. 1996. Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics. Molecular and General Genetics 250(4): 405–413.

  • D’Hont, Angélique, David Ison, Karine Alix, Catherine Roux, and Jean Christophe Glaszmann. 1998. Determination of basic chromosome numbers in the genus Saccharum by physical mapping of ribosomal RNA genes. Genome 41(2): 221–225.

    Article  Google Scholar 

  • D’Hont, Angélique, Florence Paulet, and Jean Christophe Glaszmann. 2002. Oligoclonal interspecific origin of ‘North Indian’and ‘Chinese’ sugarcanes. Chromosome Research 10(3): 253–262.

    Article  PubMed  Google Scholar 

  • D’Hont, A. 2005. Unraveling the genome structure of polyploids using FISH and GISH; examples of sugarcane and banana. Cytogenetic and Genome Research 109(1–3): 27–33.

    PubMed  Google Scholar 

  • Elgsti, OJ, and Pierre Dustin. 1955. Colchicine-in agriculture, medicine, biology and chemistry. Colchicine-in agriculture, medicine, biology and chemistry 50: 65–98.

  • Grivet, Laurent, and Paulo Arruda. 2002. Sugarcane genomics: depicting the complex genome of an important tropical crop. Current Opinion in Plant Biology 5(2): 122–127.

    Article  CAS  PubMed  Google Scholar 

  • Guerra, M., and M.J. Souza. 2002. Como observar cromossomos: um guia prático de técnicas em citogenética vegetal, animal e humana. Brasil: Editora Fumpec. 131.

  • Gupta, Vineeta, Akash Kumar, Isha Saini, and Ajit Kumar Saxena. 2013. Cytogenetic profile of aplastic anaemia in Indian children. The Indian Journal of Medical Research 137(3): 502.

    PubMed  PubMed Central  Google Scholar 

  • Jenkin, M.J., S.M. Reader, K.A. Purdie, and T.E. Miller. 1995. Detection of rDNA sites in sugarcane by FISH. Chromosome Research 3(7): 444–445.

    Article  CAS  PubMed  Google Scholar 

  • Mondin, Mateus, and Americo Docha Neto. 2006. Citogenética vegetal enfatizando a família Orchidaceae. Orchidstudium 4: 24–54.

    Google Scholar 

  • Morejohn, Louis C., and Donald E. Fosket. 1991. The biochemistry of compounds with anti-microtubule activity in plant cells. Pharmacology & Therapeutics 51(2): 217–230.

    Article  CAS  Google Scholar 

  • Panje, R.R., and C.N. Babu. 1960. Studies in Saccharum spontaneum distribution and geographical association of chromosome numbers. Cytologia 25: 152–172.

    Article  Google Scholar 

  • Perecin, D., and J.C. Barbosa. 1994. Afinidade entre distribuições de contágio e Poisson para fins práticos de amostragem. Revista de Matemática e Estatística 12: 107–112.

    Google Scholar 

  • SAS Institute (2008). SAS/STAT 9.2 user’s guide.SAS Institute, Cary, NC, USA.

  • Silvarolla, Maria Bernadete, and M.L.R. dc Aguiar-Perecin. 1994. Evaluation of chromosome number stability in two sugarcane varities. Revista Brasileira de Genética 17(2): 237–242.

    Google Scholar 

  • Tlaskal, J., and P.B. Hutchinson. 1973. The detection of mitotic instability in sugarcane. Sugarcane Breeders’ Newsletter 31: 11–16.

    Google Scholar 

Download references

Acknowledgments

This research was supported by Institutos Nacionais de Ciência e Tecnologia (INCT-CNPq n° 574002/2008-1, FAPESP n° 2008/57908-6) and IAC (Instituto Agronômico de Campinas). M. N. G. Melloni receives a Doctor Fellowship from FAPESP (2012/15281-2).

Author information

Authors and Affiliations

  1. Faculdade de Ciências Agrárias e Veterinárias da Universidade Estadual Paulista, Campus Jaboticabal, Via de acesso Prof. Donato Castellani s/n, Jaboticabal, SP, Brazil

    Maria Natália G. Melloni, Maria Letícia G. Melloni & Dilermando Perecin

  2. Centro de Cana do Instituto Agronômico de Campinas, Rodovia Antônio Duarte Nogueira, km 321 (Anel Viário Contorno Sul), Caixa Postal, 206, Ribeirão Preto, SP, Brazil

    Ana Caroline Neuber, Marcos G. de Andrade Landell & Luciana R. Pinto

Authors
  1. Maria Natália G. Melloni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria Letícia G. Melloni
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ana Caroline Neuber
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dilermando Perecin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marcos G. de Andrade Landell
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Luciana R. Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luciana R. Pinto.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Melloni, M.N.G., Melloni, M.L.G., Neuber, A.C. et al. Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane. Sugar Tech 18, 222–228 (2016). https://doi.org/10.1007/s12355-015-0381-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12355-015-0381-2

Keywords

Search

Navigation