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Extracellular Matrix Protein Laminin Induces Matrix Metalloproteinase-9 in Human Breast Cancer Cell Line MCF-7

  • Original Paper
  • Published:
Cancer Microenvironment

Abstract

Studies on interaction of tumor cells with extracellular matrix (ECM) components showed increased extracellular protease activity mediated by the family of matrix metalloproteinases (MMPs). Here we studied the effect of human breast cancer cell line MCF-7-laminin (LM) interaction on MMPs and the underlying signaling pathways. Culturing of MCF-7 cells on LM coated surface upregulated MMP-9 expression as well as reduced tissue inhibitor of metalloproteinases-1 (TIMP-1) expression. LM induced MMP-9 expression is abrogated by the blockade of α2 integrin. Inhibitor studies indicate possible involvement of phosphatidyl-inositol-3-kinase (PI3K), extracellular signal regulated kinase (ERK) and nuclear factor-kappaB (NF-κB) in LM induced signaling. LM treatment also enhanced phosphorylation of FAK (focal adhesion kinase), PI3K, ERK; nuclear translocation of ERK, pERK, NF-κB and cell migration. Our findings indicate that, binding of MCF-7 cells to LM, possibly via α2β1 integrin, induces signaling involving FAK, PI3K, ERK, NF-κB followed by upregulation of MMP-9 and cell migration.

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Acknowledgments

The authors wish to express their thanks to Director, Chittaranjan National Cancer Institute, for academic, financial and infrastructural support.

Conflict of interest

The authors declare that they have no conflict of interest.

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Author notes

  1. Amitava Chatterjee

    Present address: , 15B Pubali Garden, Ramchandrapur, Narendrapur, Kolkata, 700 103, India

Authors and Affiliations

  1. Department of Receptor Biology & Tumor Metastasis, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata, 700 026, West Bengal, India

    Sekhar Pal, Shuvojit Moulik, Anindita Dutta & Amitava Chatterjee

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  1. Sekhar Pal
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  2. Shuvojit Moulik
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  3. Anindita Dutta
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Correspondence to Amitava Chatterjee.

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Pal, S., Moulik, S., Dutta, A. et al. Extracellular Matrix Protein Laminin Induces Matrix Metalloproteinase-9 in Human Breast Cancer Cell Line MCF-7. Cancer Microenvironment 7, 71–78 (2014). https://doi.org/10.1007/s12307-014-0146-6

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  • DOI: https://doi.org/10.1007/s12307-014-0146-6

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