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Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques

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Abstract

Duabanga moluccana (or locally known as sawih) is an indigenous fast growing tropical tree species that confers various advantages for the timber industry and for planted forests development. In this paper, we isolated and characterized 8 polymorphic microsatellite markers from the D. moluccana genome using ISSR-suppression PCR techniques. The number of alleles and PIC values ranged from 3 to 8 alleles per locus and from 0.488 to 0.792, respectively. Three microsatellite loci were deviated from Hardy-Weinberg equilibrium (P < 0.05). The transferability rate ranged from 24 to 100 % among the three indigenous tree species tested. This indicates that the newly developed microsatellite markers would be useful tools for population genetic studies on D. moluccana and other indigenous tree species.

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Acknowledgments

The authors would like to thank all the lab assistants and foresters involved in this research project for their excellent field assistance in sample collection. This work is part of the joint Industry-University Partnership Programme, a research programme funded by the Sarawak Forestry Corporation (SFC) and Universiti Malaysia Sarawak (UNIMAS) to WS Ho. This research was also supported by the Ministry of Higher Education of Malaysia (No. E14009/F07/09/680/2008(13)).

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Authors and Affiliations

  1. Forest Genomics and Informatics Laboratory (fGiL), Department of Molecular Biology, Faculty of Resource Science & Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia

    Kit-Siong Liew & Wei-Seng Ho

  2. Applied Forest Science and Industry Development (AFSID), Sarawak Forestry Corporation, 93250, Kuching, Sarawak, Malaysia

    Shek-Ling Pang & Abdullah Julaihi

Authors
  1. Kit-Siong Liew
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  2. Wei-Seng Ho
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  3. Shek-Ling Pang
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  4. Abdullah Julaihi
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Correspondence to Wei-Seng Ho.

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Liew, KS., Ho, WS., Pang, SL. et al. Development and characterization of microsatellite markers in sawih tree (Duabanga moluccana Blume) using ISSR-suppression PCR techniques. Physiol Mol Biol Plants 21, 163–165 (2015). https://doi.org/10.1007/s12298-014-0262-2

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  • DOI: https://doi.org/10.1007/s12298-014-0262-2

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