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Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

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Abstract

Apical and axillary buds of Guizotia abyssinica Cass., isolated from seedlings raised in vitro, were cultured. High frequency of shoot regeneration was achieved on MS medium with BAP (1 mgl−1). Effect of BAP, Kn and GA3 applied successively in culture on shoot regeneration and flower bud formation has been studied. The shoots differentiated in cultures elongated on this medium. These rooted subsequently on half strength MS medium. The shoots flowered in vitro on MS medium with a combination of BAP (0.1mgl−1) + GA3 (0.1 mgl−1). The plantlets thus formed were successfully hardened with 90 % survival.

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References

  • Aina O, Quesenberry K, Gallo M (2012) Photoperiod affects in vitro flowering in wild peanut Arachis paraguariensis. Am J Plant Sci 3:567–571

    Article  Google Scholar 

  • Ali A, Fletcher R (1970) Hormonal regulation of apical dominance in soybeans. Can J Bot 48:1989–1994

    Article  CAS  Google Scholar 

  • Alieni M, Kokkirala VR, Yarra R, Vemunoori AK, Kasula K, Umate P, Abbagani S (2011) In vitro regeneration flowering and seed formation from leaf explants of Scoparia dulcis L. Med Aromat Plant Sci Biotechnol 5(2):11–14

    Google Scholar 

  • Ameha M, Skirvin RM, Mitiku G, Dullock D, Hofman (1998) In vitro tendril and flower development in cucumber (Cucumis sativus) may be regulated by gibberellins. J Hortic Sci Biotechnol 73(2):159–163

    CAS  Google Scholar 

  • Arokiasamy S, Ignacimuthu S, Melchias G (2000) Influence of growth regulators and explants type on in vitro shoot propagation and rooting of red sandal wood (Pterocarpus santalinus L.). Indian J Exp Biol 38:1270–1273

    Google Scholar 

  • Asghari F, Hossieni B, Hassani A, Shirzad H (2012) Effect of explants source and different hormonal combinations on direct regeneration of basil plants (Ocimum basilicum L.). A J A E 3(1):12–17

    Google Scholar 

  • Asilbekova DT, Ulchenko NT, Rakhimova NK, Nigmatullaev AM, Glushenkova AI (2005) Seed lipids from Crotalaria alata and Guizotia abyssinica. Chem Nat Compd 41(5):596–597

    Article  CAS  Google Scholar 

  • Berlyn GP, Miksche JP (1976) Botanical microtechnique and cytochemistry IOWA. State University Press, Ames, pp 30–65

    Google Scholar 

  • Bernier G, Kinet JM, Sachs RM (1981) The physiology of flowering, vol. II. Transition to reproductive growth. CRC Press, Boca Raton

    Google Scholar 

  • Bhandari HR, Sujatha M, Dangi KS, Sivasankar A (2009) Efficient plantlet regeneration from seedling explants of niger (Guizotia abyssinica Cass). Int J Agric Environ Biotechnol 2(4):438–444

    Google Scholar 

  • Bhat JG, Murthy HN (2008) Haploid plant regeneration from unpollinated ovule cultures of Niger (Guizotia abyssinica (L. f.) Cass.). Russ J Plant Physiol 55(2):241–245

    Article  CAS  Google Scholar 

  • Buising CM, Shoemaker RC, Bendrw RM (1994) Early events of multiple bud formation and shoot development in soyabean embryonic axes treated with cytokinin 6- benzylaminopurne. Am J Bot 81(11):1435–1448

    Article  CAS  Google Scholar 

  • Chakraborty D, Mandal AKA, Datta SK (2000) Retrieval of new coloured chrysanthemum through organogenesis from Sectorial chimera. Curr Sci 789:1060–1061

    Google Scholar 

  • Chang WC, Hsing YE (1980) In vitro flowering of embryoids derived from mature root callus of ginseng (Panax ginseng). Nature 284:341–342

    Article  Google Scholar 

  • Erdag BB, Emek YC (2009) Adventitious shoot regeneration and in vitro flowering of Anthemis xylopoda O. Schwarz, a critically endangered Turkish endemic. Turk J Biol 33:319–326

    CAS  Google Scholar 

  • Ericksson ME, Israelsson M, Olssono O, Moritz T (2000) Increased gibberellin biosynthesis in transgenic trees promotes growth, biomass production and xylene fiber length. Nat Biotechnol 18:784–788

    Article  Google Scholar 

  • Fukuda H, Komamine A (1985) Cyto differentiation. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. Academic Orlando, Orlando

    Google Scholar 

  • Gailite A, Klavina D, Ievinsh G (2010) In vitro propagation of an endangered plant Saussurea esthonica. Environ Exp Biol 8:43–48

    Google Scholar 

  • Galoch E, Czaplewska J, Laukajtys EB, Kopcewicz J (2002) Induction and stimulation of in vitro flowering of Pharbitis nil by cytokinin and gibberellins. Plant Growth Regul 37:199–205

    Article  CAS  Google Scholar 

  • Gomes F, Canhoto JM (2003) Micropropagation of Eucalyptus nitens Maiden (Shining gum). In Vitro Cell Dev Biol Plant 39:316–321

    Article  CAS  Google Scholar 

  • Haque SKM, Ghosh B (2013a) Micropropagation, in vitro flowering and cytological studies of Bacopa chamaedryoides an ethno-medicinal plant. Environ Exp Biol 11:59–68

    Google Scholar 

  • Haque SM, Ghosh B (2013b) In vitro completion of sexual life cycle: production of R1 plant of Ipomoea quamoclit L. Propag Ornam Plants 13:19–24

    Google Scholar 

  • Hesar AA, Kaviani B, Tarang A, Zanjani SB (2011) Effect of different concentrations of kinetin on regeneration of ten weeks (Matthiola incana). POJ 4(5):236–238

    CAS  Google Scholar 

  • Huang XL, Yang B, Hu CG, Yao JL (2009) In vitro induction of inflorescence in Dioscorea zingiberensis. Plant Cell Tissue Organ Cult 99:209–215

    Article  CAS  Google Scholar 

  • Jadimath VG, Murthy HN, Pyathi AN, Ashok Kumar HG, Ravishankar BV (1998) Plant regeneration from leaf cultures of Guizotia abyssinica (niger) and G. scabra. Phytomorphology 48:131–135

    Google Scholar 

  • Jayaseelan M (2001) In vitro studies on Cardiospermum halicacabum (L.) A medicinal herb Ph.D Thesis Bharathidasan University, Tiruchirappalli

  • Kielkowska A, Havey MJ (2012) In vitro flowering and production of viable pollen of cucumber. Plant Cell Tissue Organ Cult 109:73–82

    Article  CAS  Google Scholar 

  • Kumar MS, Rao MV (2007) In vitro micropropagation of Heliotropium indicum Linn.-An Ayurvedic herb. Indian J Biotechnol 6:245–249

    CAS  Google Scholar 

  • Lakshmi Sita G (1991) Tissue- cultured sandalwood. Curr Sci 61(12):794–795

    Google Scholar 

  • Letham DS, Palni LMS (1983) The biosynthesis and metabolism of cytokinins. Annu Rev Plant Physiol 34:163–197

    Article  CAS  Google Scholar 

  • Lin CS, Chen CT, Lin CC, Chang WC (2003) A method for inflorescence proliferation. Plant Cell Rep 21:838–843

    CAS  PubMed  Google Scholar 

  • Maheshwari P, Kumar A (2006) Organogenesis, shoot regeneration and flowering response of Vernonia cinerea to different auxin/cytokinin combinations. In Vitro Cell Dev Biol Plant 42:589–595

    Article  CAS  Google Scholar 

  • Marini F, Magrì AL, Marini D, Balestrieri F (2003) Characterization of the lipid fraction of Niger seeds (Guizotia abyssinica Cass.) from different regions of Ethiopia and India and chemometric authentication of their geographical origin. Eur J Lipid Sci Technol 105:697–704

    Article  CAS  Google Scholar 

  • Minocha SC (1987) Plant growth regulators and morphogenesis in cell and tissue culture of forest trees. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol 1. Martinus Nijhoff Publ, Dordrecht, pp 50–66

    Chapter  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Nikam TD, Shitole MG (1993) Regeneration of niger (Guizotia abyssinica Cass) cv. Sahyadri from seedling explants. Plant Cell Tissue Organ Cult 32:345–349

    Google Scholar 

  • O’Brien TP, Mccully ME (1981) The study of plant structure, principles and selected methods. Termacarphi Pvt Ltd., Melbourne, pp 4.14–4.55

    Google Scholar 

  • Piccioni E, Standardi A (1995) Encapsulation of micropropagated buds of six woody species. Plant Cell Tissue Organ Cult 42:221–226

    Article  Google Scholar 

  • Rani S, Rana JS (2010) In vitro propagation of Tylophora indica-influence of explanting season, growth regulator synergy, culture passage and planting substrate. J Am Sci 6(12):386–392

    Google Scholar 

  • Rauf S, Rahman H, Khan TM (2004) Effect of kinetin on multiple shoot induction in cotton (Gossypium hirsutum L.) cv. NIAB-999. Iran J Biotechnol 2:279–282

    CAS  Google Scholar 

  • Raven PH, Evert RF, Eichorn SE (1999) Biology of plant, vol 1, 6th edn. W. H. Freeman and Company, New York, pp 11–15

    Google Scholar 

  • Rkhis AC, Maalej M, Messaoud SO, Drira N (2006) In vitro vegetative growth and flowering of olive tree in response to GA3 treatment. Afr J Biotechnol 5(22):2097–2302

    Google Scholar 

  • Sahoo Y, Pattnaik SK, Chand PK (1997) In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (Sweet Basil) by axillary shoot proliferation. In Vitro Cell Dev Biol Plant 33:293–296

    Article  Google Scholar 

  • Sai Murali RS, Lawa Hari N, Basavaraju R (2011) In vitro morphogenic studies on Stevia rebaudiana (Bertoni) Hemsl. IUP J Genet Evol 4(1):7–14

    Google Scholar 

  • Sarvesh A, Reddy TP, Kavi Kishor PB (1996) In vitro anther culture and flowering in Guizotia abyssinica Cass. Indian J Exp Biol 34(6):565–568

    CAS  Google Scholar 

  • Sekioka TA, Tanaka JS (1981) Differentiation in callus culture of cucumber (Cucumis sativus L.). Hortic Sci 16:451 (Abstr. 386)

    Google Scholar 

  • Sharma U, Rama Rao V, Mohan JSS, Reddy AS (2007) In vitro propagation of Dendrobium microbulbon A. Rich a rare ethanomedicinal herb. Indian J Biotechnol 6:381–384

    CAS  Google Scholar 

  • Sivanesan I, Jeong BR (2007) Micropropagation and in vitro flowering in Pentanema indicum Liang. Plant Biotechnol 24:527–532

    Article  CAS  Google Scholar 

  • Stephan R, Jayabalan N (1998) In vitro flowering and seed setting formation of Coriandrum sativum L. Curr Sci 74:195–197

    Google Scholar 

  • Stockigt J, Obitz P, Falkenhagen H, Lutterbach R, Endreb S (1995) Natural products and enzymes from plant cell cultures. Plant Cell Tissue Organ Cult 43:97–109

    Article  Google Scholar 

  • Sudhakaran S, Sivasankari V (2002) In vitro flowering response of Ocimum basilicum L. J Plant Biotechnol 4(4):181–183

    Google Scholar 

  • Tisserat B, De Mason DA (1985) Occurrence and histological structure of shoots and inflorescence produced from Pheoenix dactylifera L. Plantlets in vitro. Bull Torrey Bot Club 112(1):35–42

    Article  Google Scholar 

  • Walkey DGA (1980) Production of virus-free plants by tissue culture. In: Ingram DS, Helgeson JP, Ingram DS, Helgeson JP (eds) Tissue culture methods for plant pathologists. Blackwell Scientific, UK, pp 109–119

    Google Scholar 

  • Zhang YW, Yang CF, Gituru WR, Guo YH (2008) Within-season adjustment of sex expression in females and hermaphrodites of the clonal gynodioecious herb Glechoma longituba (Lamiaceae). Ecol Res 23:873–881

    Article  Google Scholar 

  • Zivand M, Naor V (2006) Flowering of genotypes in vitro. Propag Ornam Plants 6:3–16

    Google Scholar 

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Acknowledgments

One of us (S. Baghel) acknowledges funding received under the scheme Rajiv Gandhi National Fellowship (RGNF) of University Grants Commission (UGC), New Delhi India for carrying out this research.

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  1. Plant Tissue Culture Laboratory, Department of Biological Science, R. D. University, Jabalpur, India

    S. Baghel & Y. K. Bansal

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  1. S. Baghel
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Correspondence to Y. K. Bansal.

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Baghel, S., Bansal, Y.K. Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop. Physiol Mol Biol Plants 20, 241–247 (2014). https://doi.org/10.1007/s12298-014-0229-3

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