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Association of Interleukin-6 and Myeloperoxidase with Insulin Resistance in Impaired Fasting Glucose Subjects

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Abstract

Impaired fasting glucose (IFG) is a high risk subclinical condition for the progression of type 2 diabetes mellitus and the hyperglycemia seen in this condition is because of the development of insulin resistance (IR). Obesity, inflammation, oxidative stress and many other factors have been implicated in development of IR in type 2 diabetes mellitus and its successive complications. Current study was aimed to ascertain the correlation of inflammation and oxidative stress markers [interleukin-6 (IL-6) and myeloperoxidase (MPO)] with IR in subjects with IFG. In this study, 80 subjects (40 IFG, 40 healthy controls) aged 25–45 years were selected based on their fasting plasma glucose (FPG) values and clinical history. Serum insulin, IL-6 and MPO were estimated by ELISA method and IR was calculated using Homeostatic Model Assessment Index 2 (HOMA 2) calculator. Pearson’s correlation coefficient and independent sample ‘t’ test were used for statistical analysis. IL-6 and MPO were found to be significantly elevated in IFG group and both correlates significantly with IR (r 0.413, r 0.645). Only MPO had significant correlation with FPG (r 0.388). In conclusion, the association of altered levels of IL-6 and MPO with IR are suggestive of a role of inflammation and oxidative stress in the initiation and progression of IR in individuals with IFG.

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References

  1. Borch-Johnsen K, Colagiuri S, Balkau B, Glümer C, Carstensen B, Ramachandran A, et al. Creating a pandemic of prediabetes: the proposed new diagnostic criteria for impaired fasting glycaemia. Diabetologia. 2004;47:1396–402.

    Article  CAS  PubMed  Google Scholar 

  2. Ford ES, Zhao G, Li C. Pre-diabetes and the risk for cardiovascular disease: a systematic review of the evidence. J Am Coll Cardiol. 2010;55:1310–7.

    Article  PubMed  Google Scholar 

  3. Buysschaert M, Medina JL, Bergman M, Shah A, Lonier J. Prediabetes and associated disorders. Endocrine. 2015;48(2):371–93.

    Article  CAS  PubMed  Google Scholar 

  4. Jahromi AS, Zareian P, Madani A. Association of insulin resistance with serum interleukin-6 and TNF-[alpha] levels during normal pregnancy. Biomark Insights. 2011;6:1.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Heinecke JW, Goldberg IJ. Myeloperoxidase: A therapeutic target for preventing insulin resistance and the metabolic sequelae of obesity? Diabetes. 2014;63(12):4001–3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Wegner M, Araszkiewicz A, Stolzmann MP, Wysocka BW, Ziolkiewicz DZ. Association between IL-6 concentration and diabetes-related variables in DM1 patients with and without microvascular complications. Inflammation. 2013;36(3):723–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Konukoglu D, Hatemi H, Bayer H, Bağriaçik N. Relationship between serum concentrations of interleukin-6 and tumor necrosis factor alpha in female Turkish subjects with normal and impaired glucose tolerance. Horm Metab Res. 2006;38(1):34–7.

    Article  CAS  PubMed  Google Scholar 

  8. Cardellini M, Andreozzi F, Laratta E, Marini MA, Lauro R, Hribal ML, et al. Plasma interleukin-6 levels are increased in subjects with impaired glucose tolerance but not in those with impaired fasting glucose in a cohort of Italian Caucasians. Diabetes Metab Res Rev. 2007;23(2):141–5.

    Article  CAS  PubMed  Google Scholar 

  9. Choi KM, Lee J, Lee KW, Seo JA, Oh JH, Kim SG, et al. Comparison of serum concentrations of C-reactive protein, TNF-α, and interleukin 6 between elderly Korean women with normal and impaired glucose tolerance. Diabetes Res Clin Pract. 2004;64(2):99–106.

    Article  CAS  PubMed  Google Scholar 

  10. Urakawa H, Katsuki A, Sumida Y, Gabazza EC, Murashima S, Morioka K, et al. Oxidative stress is associated with adiposity and insulin resistance in men. J Clin Endocrinol Metab. 2003;88(10):4673–6.

    Article  CAS  PubMed  Google Scholar 

  11. Gopaul NK, Manraj MD, Hebe A, Yan SL, Johnston A, Carrier MJ, et al. Oxidative stress could precede endothelial dysfunction and insulin resistance in Indian Mauritians with impaired glucose metabolism. Diabetologia. 2001;44(6):706–12.

    Article  CAS  PubMed  Google Scholar 

  12. Andrade VL, Petruceli E, Belo VA, Andrade-Fernandes CM, Russi CV, Bosco AA, et al. Evaluation of plasmatic MMP-8, MMP-9, TIMP-1 and MPO levels in obese and lean women. Clin Biochem. 2012;45(6):412–5.

    Article  CAS  PubMed  Google Scholar 

  13. Meigs JB, Larson MG, Fox CS, Keaney JF, Vasan RS, Benjamin EJ. Association of oxidative stress, insulin resistance, and diabetes risk phenotypes the Framingham offspring study. Diabetes Care. 2007;30(10):2529–35.

    Article  CAS  PubMed  Google Scholar 

  14. Ferrannini E. Definition of intervention points in prediabetes. Lancet Diabetes Endocrinol. 2014;2(8):667–75.

    Article  PubMed  Google Scholar 

  15. Garg JP, Bakris GL. Microalbuminuria: marker of vascular dysfunction, risk factor for cardiovascular disease. Vasc Med. 2002;7:35–43.

    Article  PubMed  Google Scholar 

  16. Gómez García A, Rodríguez MR, Gómez Alonso C, Ochoa DY, Alvarez Aguilar C. Myeloperoxidase is associated with insulin resistance and inflammation in overweight subjects with first-degree relatives with type 2 diabetes mellitus. Diabetes Metab J. 2015;39(1):59–65.

    Article  PubMed  PubMed Central  Google Scholar 

  17. Fonseca VA. Early identification and treatment of insulin resistance: impact on subsequent prediabetes and type 2 diabetes. Clin Cornerstone. 2007;8(Suppl 7):S7–18.

    Article  PubMed  Google Scholar 

  18. Kim JH, Bachmann RA, Chen J. Interleukin-6 and insulin resistance. Vitam Horm. 2009;80:613–33.

    Article  CAS  PubMed  Google Scholar 

  19. Styskal J, Van Remmen H, Richardson A, Salmon AB. Oxidative stress and diabetes: What can we learn about insulin resistance from antioxidant mutant mouse models? Free Radic Biol Med. 2012;52(1):46–58.

    Article  CAS  PubMed  Google Scholar 

  20. Marcovecchio ML, Dalton RN, Prevost AT, Acerini CL, Barrett TG, Cooper JD, et al. Prevalence of abnormal lipid profiles and the relationship with the development of microalbuminuria in adolescents with type 1 diabetes. Diabetes Care. 2009;32:658–63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Nevado J, Peiró C, Vallejo S, El-Assar M, Lafuente N, Matesanz N, et al. Amadori adducts activate nuclear factor-kappa β-related proinflammatory genes in cultured human peritoneal mesothelial cells. Br J Pharmacol. 2005;146:268–79.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Hossain M, Faruque MO, Kabir G, Hassan N, Sikdar D, Nahar Q, et al. Association of serum TNF-α and IL-6 with insulin secretion and insulin resistance in IFG and IGT subjects in a Bangladeshi population. Int J Diabetes Mellit. 2010;2:165–8.

    Article  CAS  Google Scholar 

  23. Van der Zwan LP, Scheffer PG, Dekker JM, Stehouwer CD, Heine RJ, Teerlink T. Hyperglycemia and oxidative stress strengthen the association between myeloperoxidase and blood pressure. Hypertension. 2010;55(6):1366–72.

    Article  PubMed  Google Scholar 

  24. Pickup JC, Crook MA. Is type II diabetes mellitus a disease of the innate immune system? Diabetologia. 1998;41:1241–8.

    Article  CAS  PubMed  Google Scholar 

  25. Laakso M, Sarlund H, Salonen R, Suhonen M, Pyörälä K, Salonen JT, et al. Asymptomatic atherosclerosis and insulin resistance. Arterioscler Thromb. 1991;11:1068–76.

    Article  CAS  PubMed  Google Scholar 

  26. Vozarova B, Weyer C, Hanson K, Tataranni PA, Bogardus C, Pratley RE. Circulating interleukin-6 in relation to adiposity, insulin action, and insulin secretion. Obes Res. 2001;9(7):414–7.

    Article  CAS  PubMed  Google Scholar 

  27. Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Are oxidative stress activated signaling pathways mediators of insulin resistance and β-cell dysfunction? Diabetes. 2003;52:1–8.

    Article  CAS  PubMed  Google Scholar 

  28. Robertson RP, Harmon J, Tran PO, Tanaka Y, Takahashi H. Glucose toxicity in β-cells: type 2 diabetes, good radicals gone bad, and the glutathione connection. Diabetes. 2003;52:581–7.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We acknowledge gratefully the financial support received by Research Society for Study of Diabetes in India (RSSDI) and Manipal University (MU) in the form of research grant.

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Authors and Affiliations

  1. Department of Biochemistry, Center for Basic Sciences, Kasturba Medical College (Manipal University), Mangalore, Karnataka, 575004, India

    Ashish Agarwal, Anupama Hegde, Charu Yadav, Afzal Ahmad, Poornima Ajay Manjrekar & Rukmini Mysore Srikantiah

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  1. Ashish Agarwal
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  2. Anupama Hegde
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  3. Charu Yadav
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  4. Afzal Ahmad
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Correspondence to Anupama Hegde.

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The authors declare that they have no conflict of interest.

Ethical Standard

The study has been reviewed by an institutional review board, and has been performed in accordance with ethical standards laid down in an appropriate version of the 1964 of the Helsinki declaration.

Human and Animal Rights

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2008 (5).

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Informed consent was obtained from all patients for being included in the study.

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Agarwal, A., Hegde, A., Yadav, C. et al. Association of Interleukin-6 and Myeloperoxidase with Insulin Resistance in Impaired Fasting Glucose Subjects. Ind J Clin Biochem 32, 33–38 (2017). https://doi.org/10.1007/s12291-016-0567-8

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  • DOI: https://doi.org/10.1007/s12291-016-0567-8

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