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Usage of Conventional PCR Technology for the Detection of HLA-B27 Allele: A Significant Molecular Marker of Ankylosing Spondylitis

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Abstract

Ankylosing spondylitis is a chronic inflammatory disease that has been linked to the human leukocyte antigen class I allele HLA-B27. More than 90 % of patients with ankylosing spondylitis possess the HLA-B27 allele, but only 1 % of people with HLA-B27 develop the disease. Ankylosing spondylitis predominately affects young males. The present study was planned to find out the involvement of HLA-B27 specific allele in relation to age and sex in symptomatic suspected patients of ankylosing spondylitis using conventional PCR technology. Forty symptomatic patients of ankylosing spondylitis were included in the present study. SSP–PCR technique was used to detect the HLA-B27 specific allele. This study showed (out of 40 symptomatic suspected cases of ankylosing spondylitis only 12 patients were detected positive with HLA-B27 allele, while remaining 28 were negative) that the positivity rate for ankylosing spondylitis with HLA-B27 allele is low. Furthermore, it was observed that the males above 50 years are more prone to develop AS with HLA-B27 specific allele. It could be concluded that the conventional PCR technology is a rapid, sensitive, and confirmatory method for the diagnosis of ankylosing spondylitis.

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References

  1. Rudwaleit M. New approaches to diagnosis and classification of axial and peripheral spondylarthritis. Curr Opin Rheumatol. 2010;22:375–80.

    Article  PubMed  Google Scholar 

  2. Boyle L, Goodall J, Opat S, Gaston JS. The recognition of HLA-B27 by human CD4+ lymphocytes. J Immunol. 2001;167:2619–24.

    PubMed  CAS  Google Scholar 

  3. Akkoc N, Khan MA. Etiopathogenic role of HLA-B27 alleles in ankylosing spondylitis. APLAR J Rheumatol. 2005;8:146–53.

    Article  Google Scholar 

  4. Maksymowych WP, Crowther SM, Dhillon SS, Conner-Spady B, Lambert RG. Systematic assessment of inflammation by magnetic resonance imaging in the posterior elements of the spine in ankylosing spondylitis. Arthritis Care Res. 2010;62:4–10.

    Article  Google Scholar 

  5. Appel H, Kuhne M, Spiekermann S, Kohler D, Zacher J, Stein H, Sieper J, Loddenkemper C. Immunohistochemical analysis of hip arthritis in ankylosing spondylitis: evaluation of the bonecartilage interface and subchondral bone marrow. Arthritis Rheum. 2006;54:1805–13.

    Article  PubMed  Google Scholar 

  6. Brionez TF, Reveille JD. The contribution of genes outside the major histocompatibility complex to susceptibility to ankylosing spondylitis. Curr Opin Rheumatol. 2008;20(4):384–91.

    Article  PubMed  CAS  Google Scholar 

  7. Wilfinger WW, Mackey K, Chomczyski P. Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. Biotechniques. 1997;22:474–81.

    PubMed  CAS  Google Scholar 

  8. Williams JF. Optimization strategies for the polymerase chain reaction. Biotechniques. 1989; 762:7.

    Google Scholar 

  9. Oostveen J, Prevo R, den Boer J, van de Laar M. Early detection of sacroiliitis on magnetic resonance imaging and subsequent development of sacroiliitis on plain radiography. A prospective, longitudinal study. J Rheumatol. 1999;26:1953–8.

    PubMed  CAS  Google Scholar 

  10. Collantes-Estevez E. Assessment of 2 systems of spondyloarthropathy diagnostic and classification criteria (Amor and ESSG) by a Spanish multicenter study. European Spondyloarthropathy Study Group. J Rheumatol. 1995;22:246–51.

    CAS  Google Scholar 

  11. Allen R, Bowness P, McMichael AJ. The Role of HLA-B27 in Spondylarthritis. Immunogenetics. 1999;50:220–7.

    Article  PubMed  CAS  Google Scholar 

  12. McGinnis MD, Conrad MP, Bouwens AGM, Tilanus MGJ, Kronick MN. Automated, solid-phase sequencing of DRB region genes using T7 sequencing chemistry and dye-labeled primers. Tissue Antigens. 1995;46:173–9.

    Article  PubMed  CAS  Google Scholar 

  13. Hill AV, Jepson A, Plebanski M, Gilbert SL. Genetic analysis of host–parasite co-evolution in human malaria. Philos Trans R Soc Lond B Biol Sci. 1997;352:1317–25.

    Article  PubMed  CAS  Google Scholar 

  14. Li F, Bulbul R, Schumacher HR Jr. Kieber-Emmons T, Callegari PE, von- Feldt JM. Molecular detection of bacterial DNA in venereal associated arthritis. Arthritis Rheum. 1996;39:950–8.

    Article  PubMed  CAS  Google Scholar 

  15. Neumuller J, Schwartz DWM, Dauber E. Evaluation of four monoclonal antibodies against HLA-B27 for their reliability in HLA-B27 typing with flow cytometry (FC): comparison with the classic microlymphocytotoxic test. Cytometry. 1996;26:209–15.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to Hon. Chairman, Shri Guru Ram Rai Education Mission for all his support and guidance in pursuing such molecular diagnostic studies in our laboratory.

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Authors and Affiliations

  1. Molecular Research Laboratory, Biochemistry Department, Shri Guru Ram Rai Institute of Medical & Health Sciences, Patel Nagar, Dehradun, Uttarakhand, India

    Narotam Sharma, Tariq Masood, Satish Chandra Nautiyal, Shivani Sailwal, Rajesh K. Singh, Rajeev K. Kushwaha & R. K. Singh

  2. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, 304022, Rajasthan, India

    Veena Sharma

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  1. Narotam Sharma
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  2. Veena Sharma
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  3. Tariq Masood
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  4. Satish Chandra Nautiyal
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  5. Shivani Sailwal
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  6. Rajesh K. Singh
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  7. Rajeev K. Kushwaha
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  8. R. K. Singh
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Correspondence to Narotam Sharma.

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Sharma, N., Sharma, V., Masood, T. et al. Usage of Conventional PCR Technology for the Detection of HLA-B27 Allele: A Significant Molecular Marker of Ankylosing Spondylitis. Ind J Clin Biochem 28, 189–192 (2013). https://doi.org/10.1007/s12291-012-0261-4

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  • DOI: https://doi.org/10.1007/s12291-012-0261-4

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