Abstract
Various zygomycota species were detected during a study of the mycobiota of meju, a brick of dried fermented soybeans, used in Korean cuisine. Two hundred and sixty-eight strains were isolated from 98 finished meju products collected in various regions of Korea from 2009 to 2011, and 96 strains were isolated from in-process meju on various farms from 2010 to 2011. The isolated zygomycota were identified using phenotypic characteristics combined with DNA sequences of the internal transcribed spacer regions of ribosomal DNA and the D1/D2 nuclear ribosomal large subunit. Of 364 zygomycota strains, 108 were identified as Mucor circinelloides, 96 as M. racemosus, 60 as Lichtheimia ramosa, 22 as Rhizopus stolonifer, 16 as Lichtheimia corymbifera, and the other 62 strains comprised 10 other species. The psychrotrophic species, Mucor circinelloides and M. racemosus were predominantly present during low temperature fermentation (LTF) and the thermotolerant species Lichtheimia ramosa and Rhizomucor species were predominant during high temperature fermentation (HTF). The results suggest that temperature has a large influence on the zygomycota composition during the fermentation process of meju.
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Abe, A., Oda, Y., Asano, K., and Sone, T. 2006. The molecular phylogeny of the genus Rhizopus based on rDNA sequences. Biosci. Biotechnol. Biochem. 70, 2387–2393.
Abe, A., Asano, K., and Sone, T. 2010. A molecular phylogeny-based taxonomy of the genus Rhizopus. Biosci. Biotechnol. Biochem. 74, 1325–1331.
Alastruey-Izquierdo, A., Hoffmann, K., de Hoog, G.S., Rodriquez-Tudela, J.L., Voigt, K., Bibashi, E., and Walther, G. 2010. Species recognition and clinical relevance of the zygomycetous genus Lichtheimia (syn. Absidia pro parte, Mycocladus). J. Clin. Microbiol. 48, 2154–2170.
Alvarez, E., Cano, J., Stchigel, A.M., Sutton, D.A., Fothergill, A.W., Salas, V., Rinaldi, M.G., and Guarro, J. 2011. Two new species of Mucor from clinical samples. Med. Mycol. 49, 62–72.
Benjamin, C.R. and Hesseltine, C.W. 1959. Studies on the genus Phycomyces. Mycologia 51, 751–771.
Detroy, R.W. and Hesseltine, C.W. 1969. Transformation of Aflatoxin B1 by steroid hydroxylating fungi. Can. J. Microbiol. 15, 495–500.
Domsch, K.H., Gams, W., and Anderson, T.H. 1993. Compendium of soil fungi. IHW-Verlag.
Dijksterhuis, J. and Samson, R.A. 2006. Zygomycetes. pp. 415–436. In Blackburn, C.W. (ed.). Food spoilage microorganisms. Woodhead Publishing Ltd. Cambridge, England.
Han, B.Z., Kuijpers, A.F.A., Thana, N.V., and Nout, M.J.R. 2004. Mucoraceous moulds involved in the commercial fermentation of Sufu Pehtze. Antonie van Leeuwenhoek 85, 253–257.
Hesseltine, C.W., Featherston, C.I., Lombard, G.L., and Dowell, V.R. 1985. Anaerobic growth of molds isolated from fermentation starters used for foods in Asian countries. Mycologia 77, 390–400.
Hideko, I., Okuyama, H., Ikezawa, H., and Tejima, S. 1975. Studies on lipase from Mucor javanicus. 1. Purification and properties. Biochem. Biophys. Acta. 388, 413–422.
Hong, S.B., Kim, D.H., Lee, M.A., Baek, S.Y., Kwon, S.W., and Samson, R.A. 2011. Taxonomy of Eurotium species isolated from meju. J. Microbiol. 49, 669–674.
Hoog, G.S. de, Guarro, J., Gene, J., and Figueras, M.J. 2000. Atlas of clinical fungi. 2nd ed. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands, Universitat Rovira i Virgili, Reus, Spain.
Kim, D.H., Lee, M.A., Baek, S.Y., Lee, J.K., Samson, R.A., and Hong, S.B. 2012. Identification and extracellular enzyme activities of Penicillium strains isolated from Meju. J. Microbiol. (in press).
Lee, S.S., Park, K.H., Choi, K.J., and Won, S.A. 1993. Identification and isolation of zygomycetous fungi found on maeju, a raw material of Korean traditional soysources. Kor. J. Mycol. 21, 172–187.
Lee, S.S., Sung, C.K., Yu, G.W., Oh, C.H., and Yu, H.G. 1995. Studies on safety and classification of microorganisms related to Korean traditional meju. pp. 391–464. In Yu, J.Y. (ed.). Study on the commercial scale production of meju for Korean fermented soybean products. Research report of Ministry of Science and Technology. Korea.
Lee, S.S. 1995. Meju fermentation for a raw material of Korean traditional soy products. Kor. J. Mycol. 23, 161–175.
Schipper, M.A.A. 1973. A study on variability in Mucor hiemalis and related species. Stud. Mycol. 4, 1–40.
Schipper, M.A.A. 1976. On Mucor circinelloides, Mucor racemosus and related species. Stud. Mycol. 12, 1–40.
Sung, S.C. 1965. Cup-plate assay of proteinase, lipase and phosphatase of Mucor. Bull. Inst. Chem. Acad. Sinica 10, 69–74.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28, 2731–2739.
White, T.J., Bruns, T., Lee, S.B., and Taylor, J. 1990. Amplification and direct sequencing of fungus ribosomal RNA genes for phylogenetics. pp. 315–322, In Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J. (eds.), PCR protocols, a guide to methods and applications, Academic press, San Diego, USA.
Wood-Baker, A. 1955. Effects of oxygen-nitrogen mixtures on the spore germination of mucoraceus mould. Trans. Br. Mycol. Soc. 38, 291–297.
Yihn, H.J. and Lee, B.H. 1968. Taxonomical studies of Rhizopus spp. in Korea. — Rhizopus spp. isolated from meju. Kor. J. Microbiol. 6, 100–105.
Zheng, R.Y., Chen, G.Q., Huang, H., and Liu, X.Y. 2007. A monograph of Rhizopus. Sydowia. 59, 273–372.
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Hong, SB., Kim, DH., Lee, M. et al. Zygomycota associated with traditional meju, a fermented soybean starting material for soy sauce and soybean paste. J Microbiol. 50, 386–393 (2012). https://doi.org/10.1007/s12275-012-1437-6
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DOI: https://doi.org/10.1007/s12275-012-1437-6