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Oceanobacillus manasiensis sp. nov., a moderately halophilic bacterium isolated from the salt lakes of Xinjiang, China

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Abstract

Three Gram reaction positive, rod-shaped, moderately motile halophilic bacterial strains, designated YD3-56T, YD16, and YH29, were isolated from the sediments of Manasi and Aiding salt lakes in the Xinjiang region of China, respectively. The strains grew optimally at 30–37°C, pH 8–11, in the presence of 5–10% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were closely related to members of the genus Oceanobacillus, exhibiting 99.1–99.2% similarity to O. kapialis KCTC 13177T, 99.2–99.3% to O. picturae KCTC 3821T, and 94.2–96% sequence similarity to other described Oceanobacillus species. SDS-PAGE of whole cell proteins preparations demonstrated that the strains exhibited high similarity to each other, but distinguished from O. kapialis KCTC 13177T and O. picturae KCTC 3821T (75%). DNA-DNA hybridization revealed that the similarity between the representative strain YD3-56T and O. kapialis KCTC 13177T was 35.3%, and the similarity between YD3-56T and O. picturae KCTC 3821T was 22.3%. Chemotaxonomic analysis of the strains showed menaquinone-7 was the predominant respiratory quinine. Major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipid pattern for strain YD3-56T predominantly contained phosphatidylcholine, and trace to moderate amounts of phosphatidyl ethanolamine and hydroxy-phosphatidyl ethanolamine. The diamino acid in murein was meso-diaminopimelic acid. The DNA G+C content of the strains was 39.7–40.1 mol%. On the basis of these results, the three strains should be classified as a novel species of the genus Oceanobacillus, for which the name Oceanobacillus manasiensis sp. nov. has been proposed, with the type strain as YD3-56T (=CGMCC 1.9105T =NBRC 105903T).

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Authors and Affiliations

  1. State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, 100193, P. R. China

    Lei Wang & San-Feng Chen

  2. National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing, 100088, P. R. China

    Wen-Yan Liu

  3. Key Laboratory for Agro-Microbial Resource and Application, Ministry of Agriculture, Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, 100193, P. R. China

    Lei Wang, Wen-Yan Liu, Zhi-Jing Gu, San-Feng Chen & Su-Sheng Yang

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  1. Lei Wang
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  2. Wen-Yan Liu
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Correspondence to Su-Sheng Yang.

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Wang, L., Liu, WY., Gu, ZJ. et al. Oceanobacillus manasiensis sp. nov., a moderately halophilic bacterium isolated from the salt lakes of Xinjiang, China. J Microbiol. 48, 312–317 (2010). https://doi.org/10.1007/s12275-010-0135-5

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