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Genome-wide transcriptional responses to sulfite in Saccharomyces cerevisiae

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Abstract

Sulfite is a commonly used preservative in foods, beverages, and Pharmaceuticals because it is toxic to many microorganisms. In order to understand the global response of Saccharomyces cerevisiae to sulfite, genome-wide transcript profiling following sulfite exposure was obtained. The transcription levels of 21 genes were increased more than 2-fold, while those of 37 genes decreased to a similar extent. Genes involved in carbohydrate metabolism represented the highest proportion of induced genes, which may account for the easily acquired resistance to sulfite. Most of down-regulated genes are involved in transcription, protein biosynthesis, and cell growth. The down-regulation of these genes is thought to reflect growth arrest which occurs during sulfite treatment, allowing cells to save energy. Cells treated with sulfite generated more than 70% of acetaldehyde than untreated cells, suggesting that the increased acetaldehyde production is correlated with the induction of PDC1 gene encoding pyruvate decarboxylase.

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  1. Division of Applied Biological Sciences, Sunmoon University, Asan, 336-708, Republic of Korea

    Hoon Park & Yoon-Sun Hwang

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  1. Hoon Park
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  2. Yoon-Sun Hwang
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Correspondence to Hoon Park.

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Park, H., Hwang, YS. Genome-wide transcriptional responses to sulfite in Saccharomyces cerevisiae . J Microbiol. 46, 542–548 (2008). https://doi.org/10.1007/s12275-008-0053-y

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