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Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity

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Abstract

The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related diseases. The influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of nanoparticles. Various physico-chemical parameters play a role in the interaction of proteins and nanoparticles in solution, thus influencing the disaggregation of preformed fibrils. We have examined the influence of two kinds of metallic nanoparticles on lysozyme amyloid fibrils using a multi-technique approach and focalized their impact on cytotoxicity on human neuroblastoma cells (SH-SY5Y). In particular, fluorescence, infrared and circular dichroism spectroscopies, optical and atomic force microscopy experiments have been carried out; the results are analyzed to rationalize the effects of these complexes on neural cell viability. It is remarkable, that the fibrils in the presence of AuNPs, unlike fibrils alone or with AgNPs, do not generate a significant cytotoxic effect even at high concentration and an amyloid degradation effect is visible.

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Acknowledgements

This work was partially supported by Slovak grand agency VEGA 2/0145/17, APVV-18-0284, Italian flagship NANOMAX, N-CHEM, Ministery of Education, University and Research (PRIN grant 20173L7W8K). Microscopy was carried out at the SPM@ISMN facility.

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Author notes

  1. Marianna Barbalinardo, Andrea Antosova, and Marta Gambucci contributed equally to this work.

Authors and Affiliations

  1. C.N.R. - I.S.M.N, via Gobetti, 101, Bologna, 40129, Italy

    Marianna Barbalinardo, Cristiano Albonetti, Francesco Valle & Eva Bystrenova

  2. Institute of Experimental Physics, Department of Biophysics, Slovak Academy of Science, Watsonova 47, 04001, Kosice, Slovakia

    Andrea Antosova, Zuzana Bednarikova & Zuzana Gazova

  3. Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, 06123, Italy

    Marta Gambucci, Paola Sassi & Loredana Latterini

  4. Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase (CSGI), ISMN-CNR, Bologna, 40129, Italy

    Francesco Valle

  5. Department of Medical and Clinical Biochemistry, Faculty of Medicine, Safarik University, Kosice, 04001, Slovakia

    Zuzana Gazova

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  1. Marianna Barbalinardo
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The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript.

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Correspondence to Loredana Latterini or Eva Bystrenova.

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Barbalinardo, M., Antosova, A., Gambucci, M. et al. Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity. Nano Res. 13, 1081–1089 (2020). https://doi.org/10.1007/s12274-020-2748-2

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  • DOI: https://doi.org/10.1007/s12274-020-2748-2

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